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Transferrin Native Protein

Images

 
Dot Blot: Transferrin Native Protein [NBP1-97144] - Lane 1: 100ng human Albumin HRP Conjugated. Lane 2-5: serial dilution 3 fold starting. Primary Antibody: none. Secondary Antibody: none. Block: 30 min at RT.

Product Details

Summary
Reactivity MuSpecies Glossary
Applications mIF
Conjugate
Biotin
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price

Transferrin Native Protein Summary

Description
Native Mouse Transferrin

Source: Normal serum (Accession #:AAB22049.1)

Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Preparation
Method
This product was prepared from normal serum by a multi-step process which includes delipidation and selective precipitation. It is provided in 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH: 7.6. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Mouse Transferrin and anti-Mouse Serum.
Protein/Peptide Type
Native Protein
Gene
TF
Purity
Multi-step

Applications/Dilutions

Dilutions
  • Dot Blot
Application Notes
This product is a suitable protein for use in Western Blotting and ELISA experiments. For blotting, the expected molecular weight of Mouse Transferrin is approximately 80kDa.

Packaging, Storage & Formulations

Storage
Store lyophilized antibody at 4C in the dark. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.6, 15 mg/ml Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative
0.01% Sodium Azide
Concentration
LYOPH
Purity
Multi-step
Reconstitution Instructions
Reconstitute with 500 ul deionized water

Alternate Names for Transferrin Native Protein

  • Beta-1 metal-binding globulin
  • DKFZp781D0156
  • EC 3.4.21
  • PRO1557
  • PRO2086
  • Serotransferrin
  • Siderophilin
  • TF
  • Transferrin

Background

Mouse Transferrin is a blood plasma glycoprotein which binds iron and regulates the amount of available iron in circulation. Mouse Transferrin has a high affinity for iron, but this binding is reversible. Mouse Transferrin has a molecular weight of approximately 80kDa. Mouse Transferrin also plays a role in the immune system, creating environments low in iron for which many pathogenic bacteria are unable to thrive.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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FAQs for Transferrin Native Protein (NBP1-97144). (Showing 1 - 1 of 1 FAQ).

  1. Can you confirm if product 'Transferrin Protein [Biotin] (NBP1-97144)' is validated to bind to it's cognate receptor (murine transferrin receptor)?
    • Unfortunately, we have not tested this.

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Blogs on Transferrin.

Transferrin: "Ironing" out the Details of Cellular Anemia
Transferrin is a protein found in the blood plasma, a glycoprotein that is specific for controlling free iron in the bodies' biological fluids. Transferrin has two binding sites that are specific for very tight and reversible iron binding. The binding...  Read full blog post.

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Bioinformatics

Gene Symbol TF