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Transferrin Native Protein

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SDS-Page: Transferrin Native Protein [NBP1-97006] - Mouse Transferrin Lane 1: Mouse Transferrin Load: 1.0 ug per lane Predicted/Observed size: 80 kDa, 80 kDa

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, ELISA, IHC, PAGE

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Catalog# & Conjugate Size Price

Transferrin Native Protein Summary

Description
Native Mouse Transferrin

Source: Normal serum (Accession #:Q921I1)

Store vial at 4C prior to opening. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Mouse Transferrin is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Preparation
Method
This product was prepared from normal serum by a multi-step process which includes delipidation and selective precipitation followed by extensive dialysis against the buffer stated above. Mouse Transferrin was assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse Transferrin and anti-Mouse Serum.
Protein/Peptide Type
Native Protein
Gene
TF
Purity
Multi-step

Applications/Dilutions

Dilutions
  • ELISA
  • Immunohistochemistry
  • SDS-Page
  • Western Blot
Application Notes
This product is supplied in 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH: 7.6. Mouse Transferrin has been tested by SDS-PAGE and is a suitable protein for use as a control reagent in SDS, Western Blotting, and ELISA experiments. For blotting, the expected molecular weight of Mouse Transferrin is approximately 80kDa. Mouse Transferrin, when unopened, is stable at 4C, but for extended use should be aliquoted and stored at -20C in its undiluted form. If dilutions of Mouse Transferrin are required, they should be prepared just prior to use and not retained.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH: 7.6
Preservative
No Preservative
Purity
Multi-step

Alternate Names for Transferrin Native Protein

  • Beta-1 metal-binding globulin
  • DKFZp781D0156
  • EC 3.4.21
  • PRO1557
  • PRO2086
  • Serotransferrin
  • Siderophilin
  • TF
  • Transferrin

Background

Mouse Transferrin is a blood plasma glycoprotein which binds iron and regulates the amount of available iron in circulation. Mouse Transferrin has a high affinity for iron, but this binding is reversible. Mouse Transferrin has a molecular weight of approximately 80kDa. Mouse Transferrin also plays a role in the immune system, creating environments low in iron for which many pathogenic bacteria are unable to thrive.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Blogs on Transferrin.

Transferrin: "Ironing" out the Details of Cellular Anemia
Transferrin is a protein found in the blood plasma, a glycoprotein that is specific for controlling free iron in the bodies' biological fluids. Transferrin has two binding sites that are specific for very tight and reversible iron binding. The binding...  Read full blog post.

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Bioinformatics

Gene Symbol TF
Uniprot