![Western Blot: TRAILR1/TNFRSF10A Antibody [NBP2-25092] - Western Blot Validation in Cell Lines. Loading: 15 ug of cell lysates per lane. Antibodies: DR4 NBP2-25092 (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/TRAILR1-TNFRSF10A-Antibody-Western-Blot-NBP2-25092-img0011.jpg "Western Blot: TRAILR1/TNFRSF10A Antibody [NBP2-25092] - Western Blot Validation in Cell Lines. Loading: 15 ug of cell lysates per lane. Antibodies: DR4 NBP2-25092 (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ELISA, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | Antibody was raised against a peptide corresponding to 19 amino acids near the carboxy terminus of human DR4 protein. The immunogen is located within the last 50 amino acids of DR4. Amino Acid Squence: DSGKFIYLEDGTGSAVSLE |
| Specificity | Human TRAILR1/TNFRSF10A has only 1 isoform (468aa, 50kD). TRAILR1/TNFRSF10A antibody has no cross reaction to TRAILR2/TNFRSF10B. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | TNFRSF10A |
| Purity | Peptide affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 50 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.02% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Peptide affinity purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for TRAILR1/TNFRSF10A Antibody (NBP2-25092)Find related products by research area.
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![Immunocytochemistry/Immunofluorescence: TRAILR1/TNFRSF10A Antibody [NBP2-25092] - Staining of Jurkat cells with DR4 antibody at 10 ug/mL.](http://images.novusbio.com/fullsize/TRAILR1-TNFRSF10A-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-25092-img0003.jpg "Immunocytochemistry/Immunofluorescence: TRAILR1/TNFRSF10A Antibody [NBP2-25092] - Staining of Jurkat cells with DR4 antibody at 10 ug/mL.")
![Immunohistochemistry: TRAILR1/TNFRSF10A Antibody [NBP2-25092] - Immunofluorescence Validation of DR4. Immunofluorescent analysis of 4% paraformaldehyde-fixed human spleen tissue labeling DR4 with NBP2-25092 at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody (red) and DAPI staining (blue). Image showing membrane staining on human spleen cells.](http://images.novusbio.com/fullsize/TRAILR1-TNFRSF10A-Antibody-Immunohistochemistry-NBP2-25092-img0009.jpg "Immunohistochemistry: TRAILR1/TNFRSF10A Antibody [NBP2-25092] - Immunofluorescence Validation of DR4. Immunofluorescent analysis of 4% paraformaldehyde-fixed human spleen tissue labeling DR4 with NBP2-25092 at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody (red) and DAPI staining (blue). Image showing membrane staining on human spleen cells.")
![Western Blot: TRAILR1/TNFRSF10A Antibody [NBP2-25092] - Independent Antibody Validation (IAV) via Protein Expression Profile in Cell LinesLoading: 15 ug of lysates per lane.Antibodies: DR4 NBP2-25092 (1 ug/mL), DR4 NBP1-76473 ( 4 ug/mL), and beta-actin (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate.](http://images.novusbio.com/fullsize/TRAILR1-TNFRSF10A-Antibody-Western-Blot-NBP2-25092-img0008.jpg "Western Blot: TRAILR1/TNFRSF10A Antibody [NBP2-25092] - Independent Antibody Validation (IAV) via Protein Expression Profile in Cell LinesLoading: 15 ug of lysates per lane.Antibodies: DR4 NBP2-25092 (1 ug/mL), DR4 NBP1-76473 ( 4 ug/mL), and beta-actin (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate.")
![Western Blot: TRAILR1/TNFRSF10A Antibody [NBP2-25092] - KO Validation in HeLa Cells. Loading: 10 ug of HeLa WT cell lysates or TRAILR1/TNFRSF10A KO cell lysates. Antibodies: TRAILR1/TNFRSF10A [NBP2-25092] (1 ug/ml) and beta-actin [NBP1-76692] (1 ug/ml), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/TRAILR1-TNFRSF10A-Antibody-Western-Blot-NBP2-25092-img0010.jpg "Western Blot: TRAILR1/TNFRSF10A Antibody [NBP2-25092] - KO Validation in HeLa Cells. Loading: 10 ug of HeLa WT cell lysates or TRAILR1/TNFRSF10A KO cell lysates. Antibodies: TRAILR1/TNFRSF10A [NBP2-25092] (1 ug/ml) and beta-actin [NBP1-76692] (1 ug/ml), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.")
![Immunohistochemistry: TRAILR1/TNFRSF10A Antibody [NBP2-25092] - Staining of human spleen tissue with antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/TRAILR1-TNFRSF10A-Antibody-Immunohistochemistry-NBP2-25092-img0006.jpg "Immunohistochemistry: TRAILR1/TNFRSF10A Antibody [NBP2-25092] - Staining of human spleen tissue with antibody at 5 ug/mL.")
![SDS-PAGE TNF-alpha [Unconjugated]](https://images.novusbio.com/images2/TNF-alpha_210-TA_256.jpg)
![Bioactivity TNF-alpha [Unconjugated]](https://images.novusbio.com/images2/TNFalpha_210TA_1658.jpg)
![SEC-MALS TNF-alpha [Unconjugated]](https://images.novusbio.com/images/210-ta_recombinant-human-tnf-alpha-protein-sec-mals-35202312244..jpg)
![Neutralization TRAILR3/TNFRSF10C Antibody [Unconjugated]](https://images.novusbio.com/images2/TRAIL_R3_AF630_Block_Neutralize_1507.jpg)
![Western Blot TRAILR3/TNFRSF10C Antibody [Unconjugated]](https://images.novusbio.com/images2/TRAIL_R3_AF630_Western_Blot_15786.jpg)
![Immunocytochemistry Caspase-3 Antibody [Unconjugated] - Active](https://images.novusbio.com/images2/Caspase-3_AF835_Immunocytochemistry_6532.jpg)
![Immunohistochemistry Caspase-3 Antibody [Unconjugated] - Active](https://images.novusbio.com/images2/Caspase3_AF835_Immunohistochemistry_22976.jpg)
![Immunocytochemistry Caspase-3 Antibody [Unconjugated] - Active](https://images.novusbio.com/images2/Caspase-3_AF835_Immunocytochemistry_9340.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")