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TNF-alpha Antibody (MP6-XT22) [Fluorescein]

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Mouse splenocytes (A) treated with PMA (50 ng/mL), Ca2+ Ionomycin (200 ng/mL) and Brefeldin A (5 μg/mL) for 4 hours or (B) untreated, were stained with Rat Anti-Mouse TNF-alpha CFS-conjugated Monoclonal Antibody (Catalog # IC410F) and Rat anti-Mouse CD3 APC-conjugated Monoclonal Antibody (FAB4841A). Quadrant markers were set based on isotype control antibody (IC005F). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005). Staining was performed using our protocol for Staining Intracellular Molecules.
Mouse splenocytes (A) treated with PMA (50 ng/mL), Ca2+ Ionomycin (200 ng/mL) and Brefeldin A (5 μg/mL) for 4 hours or (B) untreated, were stained with Rat Anti-Mouse TNF-alpha CFS-conjugated Monoclonal Antibody ...read more
Raw264 mouse macrophage cell line treated with 1 μg/mL LPS overnight was stained with Rat Anti-Mouse TNF-alpha Fluorescein-conjugated Monoclonal Antibody (Catalog # IC410F, filled histogram) or isotype control antibody ...read more
EL-4 mouse lymphoblast cell line treated with anti-CD3/anti-CD28, PMA, and Calcium Ionomycin were stained with Rat Anti-Mouse TNF-alpha Fluorescein-conjugated Monoclonal Antibody (Catalog # IC410F, filled histogram) or ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Flow
Clone
MP6-XT22
Clonality
Monoclonal
Host
Rat
Conjugate
Fluorescein

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
IC4101U-100UG
Alexa Fluor 405
IC4101V-100UG
Alexa Fluor 488
IC4101G-100UG
Alexa Fluor 532
FAB4101X
Alexa Fluor 594
IC4101T-100UG
Alexa Fluor 647
IC4101R-100UG
Alexa Fluor 700
IC4101N-100UG
Alexa Fluor 750
IC4101S-100UG
Allophycocyanin
FAB4101A
Biotin
FAB4101B
CoraFluor 1
FAB4101CL1
DyLight 350
FAB4101D
DyLight 405
FAB4101E
DyLight 488
FAB4101K
DyLight 550
FAB4101L
DyLight 594
FAB4101M
DyLight 650
FAB4101W
DyLight 680
FAB4101Y
DyLight 755
FAB4101Z
HRP
FAB4101H
Janelia Fluor 525
FAB4101JF525
Janelia Fluor 549
FAB4101I
Janelia Fluor 585
FAB4101JF585
Janelia Fluor 635
FAB4101JF635
Janelia Fluor 646
FAB4101J
Janelia Fluor 669
FAB4101JF669
PerCP
FAB4101C
Phycoerythrin
IC410P
Unconjugated
MAB4101
mFluor Violet 450 SE
FAB4101MFV450
mFluor Violet 500 SE
FAB4101MFV500
mFluor Violet 610 SE
FAB4101MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
MAB4101
Carrier Free
MAB4101
Carrier Free
IC410P
Carrier Free
MAB4101-SP
Carrier Free
IC4101T-100UG
Carrier Free
IC4101R-100UG
Carrier Free
IC4101G-100UG
Carrier Free
IC4101N-100UG
Carrier Free
IC4101V-100UG
Carrier Free
IC4101S-100UG
Carrier Free
IC4101U-100UG
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TNF-alpha Antibody (MP6-XT22) [Fluorescein] Summary

Immunogen
Mouse TNF-alpha
Specificity
Detects mouse TNF-alpha .
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Rat
Gene
TNF
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Intracellular Staining by Flow Cytometry 10 uL/10^6 cells
Publications
Read Publications using
IC410F in the following applications:

Packaging, Storage & Formulations

Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
Buffer
Supplied in a saline solution containing BSA and Sodium Azide.
Preservative
Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TNF-alpha Antibody (MP6-XT22) [Fluorescein]

  • APC1 protein
  • Cachectin
  • Cachetin
  • DIF
  • TNF
  • TNF, monocyte-derived
  • TNFA
  • TNF-A
  • TNFalpha
  • TNF-alpha
  • TNF-alphacachectin
  • TNFATNF, macrophage-derived
  • TNFG1F
  • TNFSF1A
  • TNFSF2
  • TNFSF2TNF superfamily, member 2
  • tumor necrosis factor (TNF superfamily, member 2)
  • tumor necrosis factor alpha
  • Tumor necrosis factor ligand superfamily member 2
  • tumor necrosis factor
  • tumor necrosis factor-alpha

Background

Tumor necrosis factor alpha (TNF-alpha, TNF- alpha , TNFA ), also known as Cachectin and TNFSF2, is the prototypic ligand of the TNF superfamily. It is a pleiotropic molecule that plays a central role in inflammation, immune system development, apoptosis, and lipid metabolism. TNF-alpha is produced by several lymphoid cells as well as by astrocytes, endothelial cells, and smooth muscle cells. Mouse TNF-alpha consists of a 35 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 179 aa extracellular domain (ECD). Within the ECD, mouse TNF-alpha shares 94% aa sequence identity with rat and 70%-77% with bovine, canine, cotton rat, equine, feline, human, porcine, and rhesus TNF-alpha. TNF-alpha is produced by a wide variety of immune, epithelial, endothelial, and tumor cells. TNF-alpha is assembled intracellularly to form a noncovalently linked homotrimer which is expressed on the cell surface. Cell surface TNF-alpha can induce the lysis of neighboring tumor cells and virus infected cells, and it can generate its own downstream cell signaling following ligation by soluble TNFR I. Shedding of membrane bound TNF-alpha by TACE/ADAM17 releases the bioactive cytokine, a 55 kDa molecular weight soluble trimer of the TNF-alpha extracellular domain. TNF-alpha binds the ubiquitous 55-60 kDa TNF RI and the hematopoietic cell-restricted 80 kDa TNF RII, both of which are also expressed as homotrimers present on virtually all cell types. Both type I and type II receptors bind TNF-alpha with comparable affinity, although only TNF RI contains a cytoplasmic death domain which triggers the activation of apoptosis. Soluble forms of both types of receptors are released and can neutralize the biological activity of TNF-alpha.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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TNF-alpha Antibody
(MP6-XT22) [Fluoresce ...
IC410F
TNF-alpha Antibody (MP6-XT22) [Fluorescein]
Species: Mu
Applications: Flow

Publications for TNF-alpha Antibody (IC410F)(2)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 2 applications: Flow Cytometry, IHC-P.


Filter By Application
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(1)
IHC-P
(1)
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Mouse
(2)
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Reviews for TNF-alpha Antibody (IC410F) (0)

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FAQs for TNF-alpha Antibody (IC410F). (Showing 1 - 4 of 4 FAQs).

  1. I need to know about TNF-alpha antibody kits for mice.
    • We currently have two TNFalpha ELISA kits specific for mouse: catalog numbers KA0257 and NBP1-92670.
  2. I would like to ask you for help. I need an antibody for Elisa to detect human TNF alpha in a supernatant from a cell culture (meaning supernatant after centrifugation of collected cell suspension from a plate well). Which of your antibodies against human tnf alpha would be suitable? I would like to buy only the primary antibody.
    • I would recommend catalog number NBP1-67821 or NB600-587; however, a full list of our anti-human TNF alpha antibodies suitable for use in ELISA can be found using this link.
  3. I am interested in a TNF alpha antibody, cross reactive for human, rat and mouse (host species: rabbit). Could your product NBP1-19532 be used in western blotting applications? Or do you have a similar product in your catalog which could fit with my request?
    • The antibody you mention, NBP1-19532, has not yet been validated in Western blot. I would instead recommend either NBP1-67821 or NB600-587. These are both rabbit polyclonal antibodies that cross-reacts with human, mouse and rat and have been used in Western blotting.
  4. Can your TNF-alpha products be used to treat TBI victims and therefore avoid the perispinal injections with Enbrel?
    • I am very sorry, but all of our products are for scientific research use only, and none are intended or approved for use in humans.

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Bioinformatics

Gene Symbol TNF