Novus Biologicals products are now on bio-techne.com

TCF4 Antibody

Images

 
Immunohistochemistry-Frozen: TCF4 Antibody [NBP1-88633] - Frozen mouse heart sections. Image submitted by a verified customer review.
Immunohistochemistry-Paraffin: TCF4 Antibody [NBP1-88633] -Staining of human cerebral cortex shows strong nuclear and cytoplasmic positivity in neurons.
Immunohistochemistry-Paraffin: TCF4 Antibody [NBP1-88633] -Staining of human testis shows moderate nuclear positivity in cells in seminiferous ducts.
Immunohistochemistry-Paraffin: TCF4 Antibody [NBP1-88633] -Staining of human duodenum shows strong nuclear positivity in glandular cells.
Immunohistochemistry-Paraffin: TCF4 Antibody [NBP1-88633] -Staining of human kidney shows strong nuclear positivity in cells in tubules.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications ICC/IF, IHC, KD, Single-Cell Western
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

TCF4 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: HGIIGPSHNGAMGGLGSGYGTGLLSANRHSLMVGTHREDGVALRGSHSLLPNQVPVPQLPVQSATSPDLNPPQDPYRGMPPGLQGQSVSSGSSEIKSDDEGDENLQD
Predicted Species
Rat (98%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TCF4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence Reported in scientific literature (PMID: 31091443)
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Frozen Validated from a verified customer review.
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Knockdown Validated Reported in scientific literature (PMID: 31091443)
  • Single Cell Western Reported in scientific literature (PMID: 31091443)
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Control Peptide
TCF4 Protein (NBP1-88633PEP)
Reviewed Applications
Read 1 Review rated 4
using
NBP1-88633 in the following applications:

Publications
Read Publications using
NBP1-88633 in the following applications:

Reactivity Notes

Mouse reactivity reported from a verified customer review.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for TCF4 Antibody

  • BHLHB19
  • bHLHb19E2-2
  • Class B basic helix-loop-helix protein 19
  • Immunoglobulin transcription factor 2
  • ITF-2
  • ITF2PTHS
  • MGC149724
  • SEF-2
  • SEF2-1
  • SEF2-1A
  • SEF2-1B
  • SEF2MGC149723
  • SL3-3 enhancer factor 2
  • TCF-4
  • transcription factor 4

Background

TCF4 encodes transcription factor 4, a basic helix-turn-helix transcription factor. The encoded protein recognizes an Ephrussi-box ('E-box') binding site ('CANNTG') - a motif first identified in immunoglobulin enhancers. This gene is expressed predominantly in pre-B-cells, although it is found in other tissues as well. Multiple alternatively spliced transcript variants that encode different proteins have been described.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

H00006934-M06
Species: Hu, Mu
Applications: ELISA, ICC/IF, IHC, IHC-P, WB
AF1329
Species: Hu, Mu, Rt
Applications: ChIP, CyTOF-ready, ICC, IHC, ICFlow, Simple Western, WB
NBP1-89679
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, WB
AF1329
Species: Hu, Mu, Rt
Applications: ChIP, CyTOF-ready, ICC, IHC, ICFlow, Simple Western, WB
NB100-91662
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
H00051176-M01
Species: Hu, Mu
Applications: ELISA, ICC/IF, KD, PLA, S-ELISA, WB
NB600-302
Species: Bv, Dr, Hu, Mu
Applications: ChIP, ELISA, Flow-IC, Flow, IB, ICC/IF, IHC, IHC-Fr, IHC-P, IP, PLA, S-ELISA, Simple Western, WB
AF6116
Species: Hu
Applications: ICC, WB
MAB7650
Species: Mu
Applications: IHC, WB
NBP2-32840
Species: Hu, Pm, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
AF4885
Species: Mu
Applications: IP, WB
AF748
Species: Hu, Mu
Applications: CyTOF-ready, Dual ISH-IHC, Flow, ICC, IHC, Simple Western, WB
H00006938-M01
Species: Hu
Applications: ELISA, ICC/IF, IHC, IHC-P, KD, WB
AF3287
Species: Hu, Mu, Rt
Applications: WB
MAB8224
Species: Mu
Applications: CyTOF-ready, ICFlow, WB
MAB8930
Species: Hu
Applications: ICC, Simple Western, WB
NBP1-47470
Species: Hu, Mu, Pm, Rt
Applications: ELISA, Flow, ICC/IF, IHC, IHC-P, Simple Western, WB
NBP2-13957
Species: Hu
Applications: IHC, IHC-P, WB

Publications for TCF4 Antibody (NBP1-88633)(3)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 4 applications: ICC/IF, IHC-P, KD, Single Cell Western.


Filter By Application
ICC/IF
(1)
IHC-P
(1)
KD
(1)
Single Cell Western
(1)
All Applications
Filter By Species
Human
(1)
Mouse
(1)
All Species
Showing Publications 1 - 3 of 3.
Publications using NBP1-88633 Applications Species
Saegusa M, Hashimura M, Kuwata T et al. Sox4 functions as a positive regulator of Beta -catenin signaling through upregulation of TCF4 during morular differentiation of endometrial carcinomas. Lab Invest 2012-04-01 [PMID: 22231735]
Tauil LH, Mader AMA, Tauil TH, Pires A. Immunohistochemistry expression of TCF4 protein on carcinoma, adenoma and non neoplastic colorectal mucosa. Journal of Coloproctology 2014-02-04 (IHC-P, Human) IHC-P Human
Kuo T, Damle M, Gonzalez BJ et al. Mesenchymal Stromal Cells Are Required for Regeneration and Homeostatic Maintenance of Skeletal Muscle Cell Rep 2019-05-14 [PMID: 31091443] (Single Cell Western, KD, ICC/IF, Mouse) Single Cell Western, KD, ICC/IF Mouse

Review for TCF4 Antibody (NBP1-88633) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NBP1-88633:
Filter by Applications
IHC
(1)
All Applications
Filter by Species
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry TCF4 NBP1-88633
Enlarge
4
reviewed by:
yidong wang
IHC Mouse 05/03/2017
View

Summary

ApplicationImmunohistochemistry
Sample TestedFrozen mouse heart sections
SpeciesMouse

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

ICC/IF Video Protocol

FAQs for TCF4 Antibody (NBP1-88633) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional TCF4 Products

Research Areas for TCF4 Antibody (NBP1-88633)

Find related products by research area.

Blogs on TCF4.

Beta Catenin in Cell Adhesion and T-cell Signaling
Beta Catenin is a cytosolic, 88 kDa intracellular protein that tightly associates with cell surface cadherin glycoproteins. It is one member of the catenin family that includes alpha Catenin, beta Catenin, and gamma Catenin. Colocalization studies usi...  Read full blog post.

Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

4
5
0
4
1
3
0
2
0
1
0

yidong wang
05/03/2017
Application: IHC
Species: Mouse

Bioinformatics

Gene Symbol TCF4
Entrez
Uniprot