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TCF-3/E2A Antibody - BSA Free

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Western Blot: TCF-3/E2A Antibody [NBP1-77119] - Human brain tissue lysate with TCF3/E2A antibody at 1 ug/mL in (A) the absence and (B) presence of peptide blocking.
Western Blot: TCF-3/E2A Antibody [NBP1-77119] - Human brain tissue lysate with TCF3/E2A antibody at 1 ug/mL in (A) the absence and (B) presence of peptide blocking.
Immunohistochemistry-Paraffin: TCF-3/E2A Antibody [NBP1-77119] - Rat liver tissue with TCF3/E2A antibody at 5 ug/mL.
Immunohistochemistry: TCF-3/E2A Antibody [NBP1-77119] - Rat brain tissue at 20 ug/mL.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP1-77119AF350
Alexa Fluor 405
NBP1-77119AF405
Alexa Fluor 488
NBP1-77119AF488
Alexa Fluor 532
NBP1-77119AF532
Alexa Fluor 594
NBP1-77119AF594
Alexa Fluor 647
NBP1-77119AF647
Alexa Fluor 700
NBP1-77119AF700
Alexa Fluor 750
NBP1-77119AF750
Biotin
NBP1-77119B
CoraFluor 1
NBP1-77119CL1
DyLight 350
NBP1-77119UV
DyLight 405
NBP1-77119V
DyLight 488
NBP1-77119G
DyLight 550
NBP1-77119R
DyLight 594
NBP1-77119DL594
DyLight 650
NBP1-77119C
DyLight 680
NBP1-77119FR
DyLight 755
NBP1-77119IR
FITC
NBP1-77119F
HRP
NBP1-77119H
Janelia Fluor 525
NBP1-77119JF525
Janelia Fluor 549
NBP1-77119JF549
Janelia Fluor 585
NBP1-77119JF585
Janelia Fluor 635
NBP1-77119JF635
Janelia Fluor 646
NBP1-77119JF646
Janelia Fluor 669
NBP1-77119JF669
PerCP
NBP1-77119PCP
mFluor Violet 450 SE
NBP1-77119MFV450
mFluor Violet 500 SE
NBP1-77119MFV500
mFluor Violet 610 SE
NBP1-77119MFV610
View Available Formulations
Catalog# & Formulation Size Price
BSA Free
NBP1-77119-0.025mg
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TCF-3/E2A Antibody - BSA Free Summary

Immunogen
Antibody was raised against a 17 amino acid synthetic peptide near the amino terminus of human TCF3. The immunogen is located within amino acids 150 - 200 of TCF3. Amino Acid Squence: LDTQPKKVRKVPPGLPS
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TCF3
Purity
Peptide affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 20 ug/mL
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Western Blot 1 ug/ml
Control Peptide
TCF-3/E2A Peptide (NBP1-77119PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Chicken (86%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Peptide affinity purified

Alternate Names for TCF-3/E2A Antibody - BSA Free

  • bHLHb21
  • bHLHb21TCF-3
  • Class B basic helix-loop-helix protein 21
  • E2A immunoglobulin enhancer-binding factor E12/E47
  • E2A
  • E2AKappa-E2-binding factor
  • Immunoglobulin enhancer-binding factor E12/E47
  • Immunoglobulin transcription factor 1
  • ITF1
  • ITF1Transcription factor ITF-1
  • Kappa-E2-binding factor
  • MGC129647
  • MGC129648
  • Pan2
  • TCF-3
  • Tcfe2a
  • transcription factor 3 (E2A immunoglobulin enhancer binding factors E12/E47)
  • Transcription factor 3
  • transcription factor E2-alpha
  • VDIR
  • VDR interacting repressor

Background

The TCF3 gene, also called E2A, encodes two basic helix-loop-helix (bHLH) transcription factors, E12 and E47, through alternative splicing. These transcription factors are involved in mediating canonical Wnt signaling, which is very important in a diverse array of cellular functions such as stem cell proliferation, self-renewal, activation, fate determination, differentiation and aging and senescence. They bind beta-catenin and can act as transcriptional activators or repressors for Wnt target genes, and have been shown to regulate specific target genes during CNS development downstream of Wnt signaling. TCF3/Lef complexes are also known to play key roles in controlling cell fate lineages in multipotent skin stem cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol TCF3
Uniprot