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TBCA Antibody

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Immunocytochemistry/Immunofluorescence: TBCA Antibody [NBP2-97322] - Immunofluorescence staining of TBCA in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human TBCA polyclonal antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Nucleus and Cytoplasm.
Immunocytochemistry/ Immunofluorescence: TBCA Antibody [NBP2-97322] - Immunofluorescence staining of TBCA in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, ...read more
Immunohistochemistry-Paraffin: TBCA Antibody [NBP2-97322] - Immunochemical staining of human TBCA in human skeletal muscle with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.
Immunohistochemistry-Paraffin: TBCA Antibody [NBP2-97322] - Immunochemical staining of human TBCA in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-97322AF350
Alexa Fluor 405
NBP2-97322AF405
Alexa Fluor 488
NBP2-97322AF488
Alexa Fluor 532
NBP2-97322AF532
Alexa Fluor 594
NBP2-97322AF594
Alexa Fluor 647
NBP2-97322AF647
Alexa Fluor 700
NBP2-97322AF700
Alexa Fluor 750
NBP2-97322AF750
Allophycocyanin
NBP2-97322APC
Biotin
NBP2-97322B
CoraFluor 1
NBP2-97322CL1
DyLight 350
NBP2-97322UV
DyLight 405
NBP2-97322V
DyLight 488
NBP2-97322G
DyLight 550
NBP2-97322R
DyLight 594
NBP2-97322DL594
DyLight 650
NBP2-97322C
DyLight 680
NBP2-97322FR
DyLight 755
NBP2-97322IR
FITC
NBP2-97322F
HRP
NBP2-97322H
Janelia Fluor 525
NBP2-97322JF525
Janelia Fluor 549
NBP2-97322JF549
Janelia Fluor 646
NBP2-97322JF646
Janelia Fluor 669
NBP2-97322JF669
PE
NBP2-97322PE
PerCP
NBP2-97322PCP
mFluor Violet 450 SE
NBP2-97322MFV450
mFluor Violet 500 SE
NBP2-97322MFV500
mFluor Violet 610 SE
NBP2-97322MFV610
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TBCA Antibody Summary

Description
This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Immunogen
Produced in rabbits immunized with E. coli-derived Human TBCA fragment.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TBCA
Purity
Antigen and protein A Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100-1:500
  • Immunohistochemistry-Paraffin 1:50-1:200

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.03% Proclin 300
Purity
Antigen and protein A Affinity-purified

Alternate Names for TBCA Antibody

  • CFA
  • chaperonin cofactor A
  • TCP1-chaperonin cofactor A
  • tubulin folding cofactor A
  • Tubulin-folding cofactor A
  • tubulin-specific chaperone a

Background

Tubulin folding cofactor A, also known as TBCA, is one of four proteins (cofactors A, D, E, and C) involved in the pathway leading to correctly folded beta-tubulin from folding intermediates. Cofactors A and D are believed to play a role in capturing and stabilizing beta-tubulin in a quasi-native confirmation. This protein is essential for cell viability and its knockdown produces a decrease in the amount of soluble tubulin, modifications in microtubules and G1 cell cycle arrest. Recombinant human TBCA protein was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol TBCA