![Western Blot: SYPL1 Antibody (EP10) [NBP2-59340] - Analysis of Human Brain showing detection of ~38 kDa Synaptophysin protein using Mouse Anti-Synaptophysin Monoclonal Antibody, Clone EP11. Lane 1: Molecular Weight Ladder (MW). Lane 2: Human Brain. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-Synaptophysin Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~38 kDa. Other Band(s): 25 kDa, 20 kDa.](http://images.novusbio.com/fullsize/SYPL1-Antibody-EP10-Western-Blot-NBP2-59340-img0008.jpg "Western Blot: SYPL1 Antibody (EP10) [NBP2-59340] - Analysis of Human Brain showing detection of ~38 kDa Synaptophysin protein using Mouse Anti-Synaptophysin Monoclonal Antibody, Clone EP11. Lane 1: Molecular Weight Ladder (MW). Lane 2: Human Brain. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-Synaptophysin Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~38 kDa. Other Band(s): 25 kDa, 20 kDa.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, IHC |
| Clone | EP10 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Immunogen | Immunoprecipitate of human brain |
| Localization | Cytoplasmic Vesicle , Secretory Vesicle , Synaptic Vesicle Membrane , Cell Junction , Synapse |
| Specificity | Detects 38kDa. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | SYPL1 |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Storage | Store at -20C. |
| Buffer | PBS (pH 7.4), 50% Glycerol |
| Preservative | 0.09% Sodium Azide |
| Purity | Protein G purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for SYPL1 Antibody (NBP2-59340)Find related products by research area.
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| Gene Symbol | SYPL1 |
![Immunohistochemistry: SYPL1 Antibody (EP10) [NBP2-59340] - Tissue: Cerebellum. Species: Human. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-Synaptophysin Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 ul for 5 minutes at RT. Localization: Neurons and fibers. Magnification: 20x.](http://images.novusbio.com/fullsize/SYPL1-Antibody-EP10-Immunohistochemistry-NBP2-59340-img0010.jpg "Immunohistochemistry: SYPL1 Antibody (EP10) [NBP2-59340] - Tissue: Cerebellum. Species: Human. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-Synaptophysin Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 ul for 5 minutes at RT. Localization: Neurons and fibers. Magnification: 20x.")
![Immunohistochemistry: SYPL1 Antibody (EP10) [NBP2-59340] - Tissue: Brain Slice. Species: Human. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-Synaptophysin Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 ul for 5 minutes at RT. Localization: Neurons and fibers but not on the white matter. Magnification: 20x.](http://images.novusbio.com/fullsize/SYPL1-Antibody-EP10-Immunohistochemistry-NBP2-59340-img0002.jpg "Immunohistochemistry: SYPL1 Antibody (EP10) [NBP2-59340] - Tissue: Brain Slice. Species: Human. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-Synaptophysin Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 ul for 5 minutes at RT. Localization: Neurons and fibers but not on the white matter. Magnification: 20x.")
![Immunohistochemistry: SYPL1 Antibody (EP10) [NBP2-59340] - Tissue: Fetal brain tissue. Species: Human. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-Synaptophysin Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 ul for 5 minutes at RT. Localization: Neurons.](http://images.novusbio.com/fullsize/SYPL1-Antibody-EP10-Immunohistochemistry-NBP2-59340-img0009.jpg "Immunohistochemistry: SYPL1 Antibody (EP10) [NBP2-59340] - Tissue: Fetal brain tissue. Species: Human. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-Synaptophysin Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 ul for 5 minutes at RT. Localization: Neurons.")
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![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")