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Rat Syndecan-1/CD138 ELISA Kit (Colorimetric)

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ELISA: Rat Syndecan-1/CD138 ELISA Kit (Colorimetric) [NBP2-76611] - Samples were spiked with high concentrations of Rat Syndecan-1/CD138 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
ELISA: Rat Syndecan-1/CD138 ELISA Kit (Colorimetric) [NBP2-76611] - Samples were spiked with high concentrations of Rat Syndecan-1/CD138 and diluted with Reference Standard & Sample Diluent to produce samples with ...read more
ELISA: Rat Syndecan-1/CD138 ELISA Kit (Colorimetric) [NBP2-76611] - Standard Curve Reference

Product Details

Summary
Reactivity RtSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and other biological fluids
Standard Curve Range
1.563 - 100 ng/mL
Sensitivity
0.938 ng/mL

Order Details

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Rat Syndecan-1/CD138 ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Rat SDC1 in samples. No significant cross-reactivity or interference between Rat SDC1 and analogues was observed.
Standard Curve Range
1.563 - 100 ng/mL
Sensitivity
0.938 ng/mL
Assay Type
Sandwich-ELISA
Inter-Assay
%CV < 4.98
Intra-Assay
%CV < 4.57
Spike Recovery
90 - 107%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
SDC1

Applications/Dilutions

Dilutions
  • ELISA
Publications
Read Publications using NBP2-76611.

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro ELISA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Stop Solution
  12. Substrate Reagent

Alternate Names for Rat Syndecan-1/CD138 ELISA Kit (Colorimetric)

  • CD138 antigen
  • CD138
  • SDC
  • SDC1
  • SYND1heparan sulfate proteoglycan fibroblast growth factor receptor
  • syndecan 1
  • syndecan proteoglycan 1
  • syndecan
  • Syndecan1
  • Syndecan-1

Background

CD138, also known as Syndecan-1, is a transmembrane heparin sulfate proteoglycan that binds type I collagen. The relative molecular mass of CD138 is found to vary between 20-95 kDa due to the presence of a variable number of GAG modifications on each CD138 molecule. This protein appears to mediate binding of cells to the extracellular matrix. Among mature tissues, CD138 is expressed on simple and stratified epithelia, endothelial cells, and fibroblasts. (2,3) While stratified keratinocytes express CD138, their malignant transformation suppresses CD138 expression. Wound repair processes alter patterns of CD138 expression. Among hematopoietic cells, CD138 is expressed by immunoglobulin-negative pre-B cells in the bone marrow, immature B cells and by terminally differentiated plasma cells in lymphoid organs. CD138 expression has not been observed on mature, circulating peripheral B lymphocytes, however. (4-7) CD138 is also strongly expressed by multiple myeloma-derived cell lines and elevated levels of CD138 are present in the serum of patients with multiple myeloma, a negative prognostic indicator for the disease. (8)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Rat Syndecan-1/CD138 ELISA Kit (Colorimetric)
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Publications for Syndecan-1/CD138 ELISA Kit (NBP2-76611)(2)

We have publications tested in 1 application: ELISA.


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  Rat Syndecan-1/CD138 ELISA Kit (Colorimetric) Manual
  PDF Datasheet
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Bioinformatics

Gene Symbol SDC1