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SUCLA2 Antibody

Images

 
Independent Antibodies: Immunohistochemistry-Paraffin: SUCLA2 Antibody [NBP2-55488] - Staining of human cerebral cortex, colon, kidney and lymph node using Anti-SUCLA2 antibody NBP2-55488 (A) shows similar ...read more
Independent Antibodies: Western Blot: SUCLA2 Antibody [NBP2-55488] - Analysis using Anti-SUCLA2 antibody NBP2-55488 (A) shows similar pattern to independent antibody NBP1-85860 (B).
Immunocytochemistry/ Immunofluorescence: SUCLA2 Antibody [NBP2-55488] - Staining of human cell line U-2 OS shows localization to mitochondria.
Immunohistochemistry-Paraffin: SUCLA2 Antibody [NBP2-55488] - Staining of human colon.
Immunohistochemistry-Paraffin: SUCLA2 Antibody [NBP2-55488] - Staining of human lymph node.
Immunohistochemistry-Paraffin: SUCLA2 Antibody [NBP2-55488] - Staining of human cerebral cortex.
Immunohistochemistry-Paraffin: SUCLA2 Antibody [NBP2-55488] - Staining of human kidney.
Simple Western: SUCLA2 Antibody [NBP2-55488] - Simple Western Lane view shows HepG2, Molt-4, U-251-MG whole cell lysate (WCL). A specific band was detected for SUCLA2 antibody (NBP2-55488) at approximately 50 kDa (as ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
 

Independent Antibodies

       

Order Details

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SUCLA2 Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to the following amino acid sequence: ATLRNHRPRTAQRAAAQVLGSSGLFNNHGLQVQQQQQRNLSLHEYMSMELLQEAGVSVPKGYVAKSPDEAYAIAKKLGSKDVVIK
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SUCLA2
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Simple Western
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
SUCLA2 Recombinant Protein Antigen (NBP2-55488PEP)

Reactivity Notes

Mouse 87%, Rat 80%

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for SUCLA2 Antibody

  • A-BETA
  • ATP-specific succinyl-CoA synthetase subunit beta
  • beta subunit
  • EC 6.2.1
  • EC 6.2.1.5
  • mitochondrial succinyl-CoA ligase [ADP-forming] subunit beta
  • MTDPS5
  • Renal carcinoma antigen NY-REN-39
  • succinate-CoA ligase, ADP-forming, beta subunit
  • succinyl-CoA ligase [ADP-forming] subunit beta, mitochondrial

Background

Succinyl-CoA synthetase (SCS) is a mitochondrial matrix enzyme that acts as a heterodimer, being composed of an invariant alpha subunit and a substrate-specific beta subunit. The protein encoded by this gene is an ATP-specific SCS beta subunit that dimerizes with the SCS alpha subunit to form SCS-A, an essential component of the tricarboxylic acid cycle. SCS-A hydrolyzes ATP to convert succinate to succinyl-CoA. Defects in this gene are a cause of myopathic mitochondrial DNA depletion syndrome. A pseudogene of this gene has been found on chromosome 6. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for SUCLA2 Antibody (NBP2-55488) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol SUCLA2