![Immunohistochemistry-Frozen: Substance P Antibody (NC1/34) [NB100-65219] - Opioid R/OPRM1 (green) and Substance P (red) were detected in frozen mouse spinal cord and DRG tissue by fluorescent multiplex IHC. Mu Opioid R/OPRM1 Antibody (NB100-1620), used at 1:100, and Substance P Antibody (NB100-65219), used at 1:50, were applied to the tissue and simultaneously incubated at 4C overnight. Anti-Rabbit Alexa Fluor(R) 488-conjugated secondary antibody and anti-Rat NL557-conjugated secondary antibody (NL013) were applied to the tissue, and simultaneously incubated at room temperature, protected from light for 1 hour. Coverslips were applied to the tissue slide along with mounting media containing DAPI (blue) and imaged using a 4X objective lens.](http://images.novusbio.com/fullsize/Substance-P-Antibody-NC1-34-Immunohistochemistry-Frozen-NB100-65219-img0001.jpg "Immunohistochemistry-Frozen: Substance P Antibody (NC1/34) [NB100-65219] - Opioid R/OPRM1 (green) and Substance P (red) were detected in frozen mouse spinal cord and DRG tissue by fluorescent multiplex IHC. Mu Opioid R/OPRM1 Antibody (NB100-1620), used at 1:100, and Substance P Antibody (NB100-65219), used at 1:50, were applied to the tissue and simultaneously incubated at 4C overnight. Anti-Rabbit Alexa Fluor(R) 488-conjugated secondary antibody and anti-Rat NL557-conjugated secondary antibody (NL013) were applied to the tissue, and simultaneously incubated at room temperature, protected from light for 1 hour. Coverslips were applied to the tissue slide along with mounting media containing DAPI (blue) and imaged using a 4X objective lens.")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | ICC/IF, IHC, IHC |
| Clone | NC1/34 |
| Clonality | Monoclonal |
| Host | Rat |
| Conjugate | Unconjugated |
| Immunogen | Substance P conjugated to Bovine Serum Albumin. |
| Specificity | NB100-65219 recognizes the COOH terminal end of substance P, a short polypeptide neurotransmitter that regulates the excitability of dorsal horn nociceptive neurons. It is known to have a role in several physiologic activities such as pain transmission, the vomiting reflex, salivary secretion and smooth muscle contraction. 5% reactivity is observed with eledoisin. It does not react with Leu or Met-enkephalin, somatostatin or beta-endorphin. |
| Isotype | IgG2a |
| Clonality | Monoclonal |
| Host | Rat |
| Gene | TAC1 |
| Purity | Tissue culture supernatant |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | This product does not require antigen retrieval prior to staining of IHC-P sections. This antibody has been used for the localization of substance P in tissue sections by immunofluorescence and PAP staining in nervous systems of experimental animals and man. Please refer to the Cuello references listed below. It has also been successfully applied in archive post-mortem tissues of the human brain, see Mai and Pioro references. Use in Immunohistochemistry whole mount reported in scientific literature (PMID 24728885) |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | Tissue culture supernatant |
| Preservative | 0.05% Thimerosal |
| Purity | Tissue culture supernatant |
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| Publication using NB100-65219 | Applications | Species |
|---|---|---|
| Pelayo JC, Veldhuis NA, Eriksson EM et al. Localisation and activation of the neurokinin 1 receptor in the enteric nervous system of the mouse distal colon. Cell Tissue Res. 2014-04-12 [PMID: 24728885] (IHC-Fr, IHC-WhMt, Mouse) | IHC-Fr, IHC-WhMt | Mouse |
| Images | Ratings | Applications | Species | Date | Details | ||||||||
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Enlarge |
reviewed by:
Giulia Di Cristina |
IHC-Fr | Other | 07/21/2016 |
Summary
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Secondary Antibodies |
Isotype Controls |
Research Areas for Substance P Antibody (NB100-65219)Find related products by research area.
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