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STAT6 [p Tyr641] Antibody (1248D) [Unconjugated]

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Western blot shows lysates of U937 human histiocytic lymphoma cell line and HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 100 ng/mL Recombinant Human IL-4 (Catalog # 204-IL) for 15 minutes or 2 ng/mL Recombinant Human IL-4 (Catalog # 204-IL) for 20 minutes, respectively. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human Phospho-STAT6 (Y641) Monoclonal Antibody (Catalog # MAB3717) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for STAT6 at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of U937 human histiocytic lymphoma cell line and HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 100 ng/mL Recombinant Human IL-4 (Catalog # 204-IL) for 15 ...read more
STAT6 phosphorylated at Y641 was detected in immersion fixed Daudi human Burkitt's lymphoma cell line, untreated (lower panel) or treated with Recombinant Human IL-4 (Catalog # 204-IL; upper panel) using Rabbit ...read more
Human peripheral blood mononuclear cells (PBMCs) either (A) Th2-stimulated or (B) unstimulated were stained with Rabbit Anti-Human Phospho-STAT6 (Y641) Monoclonal Antibody (Catalog # MAB3717) followed by anti-Rabbit IgG ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, ICC/IF
Clone
1248D
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
IC3717U-100UG
Alexa Fluor 405
IC3717V-100UG
Alexa Fluor 488
IC3717G-100UG
Alexa Fluor 594
IC3717T-100UG
Alexa Fluor 700
IC3717N-100UG
Alexa Fluor 750
IC3717S-100UG
Phycoerythrin
IC37173P-025
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB3717-SP
Carrier Free
IC37173P-025
Carrier Free
IC3717V-100UG
Carrier Free
IC3717U-100UG
Carrier Free
IC3717T-100UG
Carrier Free
IC3717S-100UG
Carrier Free
IC3717G-100UG
Carrier Free
IC3717N-100UG
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STAT6 [p Tyr641] Antibody (1248D) [Unconjugated] Summary

Additional Information
Recombinant Monoclonal Antibody.
Modification
p Tyr641
Specificity
Detects human STAT6 when phosphorylated at Y641.
Source
N/A
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
STAT6
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Applications/Dilutions

Dilutions
  • Immunocytochemistry 2-10 ug/mL
  • Intracellular Staining by Flow Cytometry 0.25 ug/10^6 cells
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
Sodium Azide
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for STAT6 [p Tyr641] Antibody (1248D) [Unconjugated]

  • D12S1644
  • EC 2.4.1.227
  • EC 2.7.7.6
  • IL-4 Stat
  • IL-4-STAT
  • signal transducer and activator of transcription 6
  • signal transducer and activator of transcription 6, interleukin-4 induced
  • STAT, interleukin-4 induced
  • STAT, interleukin4-induced
  • STAT6
  • STAT6B
  • STAT6C
  • transcription factor IL-4 STAT

Background

Signal Transducer and Activator of Transcription 6 (STAT6) mediates the signaling of cytokines such as IL-4 and IL-13. STAT6 acts as a signal transducer in the cytoplasm and, upon phosphorylation at Y641, translocates to the nucleus and binds to the DNA consensus site TTCN4GAA. Knockout studies in mice suggest that STAT6 functions in differentiation of T helper 2 (Th2) cells, expression of cell surface markers, and class switch of immunoglobulins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Isotype Controls

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Bioinformatics

Gene Symbol STAT6
Uniprot