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SSX2 Antibody (OTI4D10) - Azide and BSA Free

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Western Blot: SSX2 Antibody (OTI4D10) - Azide and BSA Free [NBP2-74368] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SSX2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SSX2.
Western Blot: SSX2 Antibody (OTI4D10) - Azide and BSA Free [NBP2-74368] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SSX2 (Right lane) cDNA for 48 hrs and lysed. Equivalent ...read more
Immunocytochemistry/ Immunofluorescence: SSX2 Antibody (OTI4D10) - Azide and BSA Free [NBP2-74368] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY SSX2.
Immunohistochemistry: SSX2 Antibody (OTI4D10) - Azide and BSA Free [NBP2-74368] - Staining of paraffin-embedded pancreas tissue using anti-SSX2 mouse monoclonal antibody.
Immunohistochemistry: SSX2 Antibody (OTI4D10) - Azide and BSA Free [NBP2-74368] - Staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-SSX2 mouse monoclonal antibody.
Immunohistochemistry: SSX2 Antibody (OTI4D10) - Azide and BSA Free [NBP2-74368] - Staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-SSX2 mouse monoclonal antibody.
Immunohistochemistry: SSX2 Antibody (OTI4D10) - Azide and BSA Free [NBP2-74368] - Staining of paraffin-embedded Carcinoma of bladder tissue using anti-SSX2 mouse monoclonal antibody.
Immunohistochemistry: SSX2 Antibody (OTI4D10) - Azide and BSA Free [NBP2-74368] - Staining of paraffin-embedded Carcinoma of thyroid tissue using anti-SSX2 mouse monoclonal antibody.
Immunohistochemistry: SSX2 Antibody (OTI4D10) - Azide and BSA Free [NBP2-74368] - Staining of paraffin-embedded colon tissue using anti-SSX2 mouse monoclonal antibody.
Immunohistochemistry: SSX2 Antibody (OTI4D10) - Azide and BSA Free [NBP2-74368] - Staining of paraffin-embedded endometrium tissue using anti-SSX2 mouse monoclonal antibody.
Immunohistochemistry: SSX2 Antibody (OTI4D10) - Azide and BSA Free [NBP2-74368] - Staining of paraffin-embedded Ovary tissue using anti-SSX2 mouse monoclonal antibody.
Immunohistochemistry: SSX2 Antibody (OTI4D10) - Azide and BSA Free [NBP2-74368] - SSX2 Antibody (4D10) [NBP1-48008

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC
Clone
OTI4D10
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-74368AF350
Alexa Fluor 405
NBP2-74368AF405
Alexa Fluor 488
NBP2-74368AF488
Alexa Fluor 532
NBP2-74368AF532
Alexa Fluor 594
NBP2-74368AF594
Alexa Fluor 647
NBP2-74368AF647
Alexa Fluor 700
NBP2-74368AF700
Alexa Fluor 750
NBP2-74368AF750
Biotin
NBP2-74368B
CoraFluor 1
NBP2-74368CL1
DyLight 350
NBP2-74368UV
DyLight 405
NBP2-74368V
DyLight 488
NBP2-74368G
DyLight 550
NBP2-74368R
DyLight 594
NBP2-74368DL594
DyLight 650
NBP2-74368C
DyLight 680
NBP2-74368FR
DyLight 755
NBP2-74368IR
FITC
NBP2-74368F
HRP
NBP2-74368H
Janelia Fluor 525
NBP2-74368JF525
Janelia Fluor 549
NBP2-74368JF549
Janelia Fluor 646
NBP2-74368JF646
Janelia Fluor 669
NBP2-74368JF669
Unconjugated
NBP1-48008
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP1-48008
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SSX2 Antibody (OTI4D10) - Azide and BSA Free Summary

Immunogen
Full-length protein expressed in 293T cell transfected with human SSX2 expression vector
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
SSX2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin
  • Western Blot 1:2000
Theoretical MW
21.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for SSX2 Antibody (OTI4D10) - Azide and BSA Free

  • Cancer/testis antigen 5.2
  • cancer/testis antigen family 5, member 2a
  • CT5.2a
  • HD21
  • HOM-MEL-40
  • MGC119055
  • MGC15364
  • MGC3884
  • protein SSX2
  • sarcoma, synovial, X-chromosome-related 2
  • SSX
  • SSX2A
  • SSX2B
  • synovial sarcoma, X breakpoint 2B
  • synovial sarcoma, X breakpoint 2CT5.2
  • Tumor antigen HOM-MEL-40

Background

The product of this gene belongs to the family of highly homologous synovial sarcoma X (SSX) breakpoint proteins. These proteins may function as transcriptional repressors. They are also capable of eliciting spontaneously humoral and cellular immune responses in cancer patients, and are potentially useful targets in cancer vaccine-based immunotherapy. SSX1, SSX2 and SSX4 genes have been involved in the t(X;18) translocation characteristically found in all synovial sarcomas. This translocation results in the fusion of the synovial sarcoma translocation gene on chromosome 18 to one of the SSX genes on chromosome X. The encoded hybrid proteins are probably responsible for transforming activity. Two transcript variants encoding distinct isoforms have been identified for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol SSX2