![Immunocytochemistry/Immunofluorescence: SSEA-1 Antibody (FUT4/1478R) - Azide and BSA Free [NBP2-49879] - Staining of U937 cells using CD15 Rabbit Recombinant Monoclonal Antibody (FUT4/1478R) followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are stained with Reddot.](http://images.novusbio.com/fullsize/SSEA-1-Antibody-FUT4-1478R-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-49879-img0002.jpg "Immunocytochemistry/Immunofluorescence: SSEA-1 Antibody (FUT4/1478R) - Azide and BSA Free [NBP2-49879] - Staining of U937 cells using CD15 Rabbit Recombinant Monoclonal Antibody (FUT4/1478R) followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are stained with Reddot.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | Flow, ICC/IF, IHC, CyTOF-ready, ICC/IF |
| Clone | FUT4/1478R |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1.0 mg/ml |
| Description | 1.0 mg/ml of antibody purified by Protein A Column. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-49878). Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Antibody is stable for 24 months. Non-hazardous. |
| Additional Information | Recombinant Monoclonal Antibody. |
| Immunogen | Recombinant human full-length SSEA-1 protein (Uniprot: P22083) |
| Localization | Cell surface and granular paranuclear |
| Marker | Reed-Sternberg Cell Marker |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | FUT4 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Application Notes | Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 1mM EDTA, pH 8.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined. |
| Theoretical MW | 220 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20 to -80C. Avoid freeze-thaw cycles. |
| Buffer | 10 mM PBS |
| Preservative | No Preservative |
| Concentration | 1.0 mg/ml |
| Purity | Protein A purified |
![Immunohistochemistry E-Selectin/CD62E Antibody (BBIG-E4) [Unconjugated]](https://images.novusbio.com/images2/E-Selectin_BBA16_Immunohistochemistry_6938.jpg)
![Immunocytochemistry E-Selectin/CD62E Antibody (BBIG-E4) [Unconjugated]](https://images.novusbio.com/images2/E-Selectin_BBA16_Immunocytochemistry_9821.jpg)
![Flow Cytometry E-Selectin/CD62E Antibody (BBIG-E4) [Unconjugated]](https://images.novusbio.com/images2/bba16_human-e-selectin-cd62e-mab-clone-bbig-e4-flow-cytometry-25102021144214.jpg)
![Immunohistochemistry CD3 Antibody (17A2) [Unconjugated]](https://images.novusbio.com/images2/CD3_MAB4841_Immunohistochemistry_7033.jpg)
![Immunocytochemistry CD3 Antibody (17A2) [Unconjugated]](https://images.novusbio.com/images2/CD3_MAB4841_Immunocytochemistry__Immunofluorescence_23528.jpg)
![Immunohistochemistry CD3 Antibody (17A2) [Unconjugated]](https://images.novusbio.com/images/antibody/mab4841_mouse-cd3-mab-clone-17a2-immunohistochemistry-88202312119.jpg)
![Flow Cytometry L-Selectin/CD62L Antibody (4G8) [Unconjugated]](https://images.novusbio.com/images/antibody/bba24_human-l-selectin-cd62l-mab-cl-4g8-flow-cytometry-822024174319..jpg)
Secondary Antibodies |
Isotype Controls |
Research Areas for SSEA-1 Antibody (NBP2-49879)Find related products by research area.
|
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | FUT4 |
![Immunohistochemistry-Paraffin: SSEA-1 Antibody (FUT4/1478R) - Azide and BSA Free [NBP2-49879] - Formalin-fixed, paraffin-embedded human Hodgkin's Lymphoma stained with CD15 Rabbit Recombinant Monoclonal Antibody (FUT4/1478R).](http://images.novusbio.com/fullsize/SSEA-1-Antibody-FUT4-1478R-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-49879-img0001.jpg "Immunohistochemistry-Paraffin: SSEA-1 Antibody (FUT4/1478R) - Azide and BSA Free [NBP2-49879] - Formalin-fixed, paraffin-embedded human Hodgkin's Lymphoma stained with CD15 Rabbit Recombinant Monoclonal Antibody (FUT4/1478R).")
![Flow Cytometry: SSEA-1 Antibody (FUT4/1478R) - Azide and BSA Free [NBP2-49879] - Analysis of U937 cells using CD15 Rabbit Recombinant Monoclonal Antibody (FUT4/1478R) followed by goat anti-rabbit IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/SSEA-1-Antibody-FUT4-1478R-Azide-and-BSA-Free-Flow-Cytometry-NBP2-49879-img0003.jpg "Flow Cytometry: SSEA-1 Antibody (FUT4/1478R) - Azide and BSA Free [NBP2-49879] - Analysis of U937 cells using CD15 Rabbit Recombinant Monoclonal Antibody (FUT4/1478R) followed by goat anti-rabbit IgG-CF488 (Blue); Isotype Control (Red).")
![Bioactivity CTLA-4 [Unconjugated]](https://images.novusbio.com/images2/CTLA4_7268CT_2293.jpg)
![Immunohistochemistry CD34 Antibody [Unconjugated]](https://images.novusbio.com/images2/CD34_AF4117_Immunohistochemistry_6824.jpg)
![Immunohistochemistry CD45 Antibody [Unconjugated]](https://images.novusbio.com/images2/CD45_AF114_Immunohistochemistry_23525.jpg)
![Immunocytochemistry CD45 Antibody [Unconjugated]](https://images.novusbio.com/images2/af114_mouse-cd45-affinity-purified-polyclonal-ab-immunocytochemistry-6122021145449.jpg)
![Immunocytochemistry/ Immunofluorescence CD45 Antibody [Unconjugated]](https://images.novusbio.com/images/af114_mouse-cd45-affinity-purified-polyclonal-ab-41202410331954.jpg)
![N/A CD14 [HRP]](https://images.novusbio.com/images2/CD14_DC140_ELISA_42.jpg)
![N/A CD14 [HRP]](https://images.novusbio.com/images2/DATA_CD14_DC140_ELISA_597.jpg)
![Siglec-3/CD33 [Unconjugated]](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "Siglec-3/CD33 [Unconjugated]")
![Chromatin Immunoprecipitation (ChIP) Oct-3/4 Antibody [Unconjugated]](https://images.novusbio.com/images2/Oct-3_4_AF1759_Chromatin_Immunoprecipitation_11218.jpg)
![Immunocytochemistry Oct-3/4 Antibody [Unconjugated]](https://images.novusbio.com/images2/Oct-3_4_AF1759_Immunocytochemistry_7093.jpg)
![Simple Western Oct-3/4 Antibody [Unconjugated]](https://images.novusbio.com/images2/Oct3_4_AF1759_Simple_Western_20183.jpg)
![Immunocytochemistry Aminopeptidase N/CD13 Antibody [Unconjugated]](https://images.novusbio.com/images2/Aminopeptidase_N_AF2335_Immunocytochemistry__Immunofluorescence_17382.jpg)
![Immunohistochemistry Aminopeptidase N/CD13 Antibody [Unconjugated]](https://images.novusbio.com/images2/Aminopeptidase_N_AF2335_Immunohistochemistry_23316.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")