Novus Biologicals products are now on bio-techne.com

Sphingomyelin Synthase 2 Antibody

Images

 
Western Blot: Sphingomyelin Synthase 2 Antibody [NBP1-92436] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp
Orthogonal Strategies: Immunohistochemistry-Paraffin: Sphingomyelin Synthase 2 Antibody [NBP1-92436] - Analysis in human gallbladder and skeletal muscle tissues. Corresponding SGMS2 RNA-seq data are presented for ...read more
Immunohistochemistry-Paraffin: Sphingomyelin Synthase 2 Antibody [NBP1-92436] - Staining of human gallbladder shows strong membranous positivity in glandular cells.
Immunohistochemistry-Paraffin: Sphingomyelin Synthase 2 Antibody [NBP1-92436] - Staining of human skeletal muscle shows weak positivity in myocytes.
Immunohistochemistry-Paraffin: Sphingomyelin Synthase 2 Antibody [NBP1-92436] - Staining of human Fallopian tube shows weak membranous positivity in glandular cells.
Immunohistochemistry-Paraffin: Sphingomyelin Synthase 2 Antibody [NBP1-92436] - Staining of human small intestine shows moderate membranous positivity in glandular cells.
Immunohistochemistry-Paraffin: Sphingomyelin Synthase 2 Antibody [NBP1-92436] - Staining of human lung shows moderate membranous positivity in pneumocytes.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

Order Details

Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Sphingomyelin Synthase 2 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: IIETAKLEEHLENQPSDPTNTYARPAEPVEEENKNGNGKPKSLSSGLRKGTKKYPDYIQIAMPTESR
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SGMS2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Control Peptide
Sphingomyelin Synthase 2 Protein (NBP1-92436PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Sphingomyelin Synthase 2 Antibody

  • EC 2.7.8.27
  • phosphatidylcholine:ceramide cholinephosphotransferase 2
  • SM synthase
  • sphingomyelin synthase 2SMS2MGC26963

Background

Sphingomyelin (SM) is a major component of plasma membranes. It is preferentially concentrated in the outer leaflet and has a role in the formation of lipid rafts. SM synthases (EC 2.7.8.27), such as SGMS2, produce SM in the lumen of the Golgi and on the cell surface through the transfer of phosphocholine from phosphatidylcholine onto ceramide, yielding diacylglycerol as a side product (Huitema et al., 2004 [PubMed 14685263]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-92435
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP2-00900
Species: Ca, Hu, Pm, Mu, Pm, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
NBP1-28745
Species: Hu
Applications: IHC, IHC-P, IP, WB
NBP2-45889
Species: Hu
Applications: IHC, IHC-Fr, IHC-P, WB
AF6868
Species: Hu
Applications: IHC, KO, WB
NBP1-87810
Species: Fi, Hu
Applications: ICC/IF, IHC, IHC-P, WB
MAB1417
Species: Bv, Hu, Mu
Applications: CyTOF-ready, ICC, IHC, ICFlow
NBP3-10064
Species: Hu
Applications: WB
NBP2-34294
Species: Hu, Mu, Rt
Applications: Flow, IHC, IHC-P
NB100-56566
Species: Bv, Hu, Ma, Mu, Po, Rt
Applications: B/N, ChIP, CyTOF-ready, DB, Dual ISH-IHC, ELISA(Cap), ELISA, Flow-CS, Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, In vitro, KD, KO, PAGE, WB
NBP1-87789
Species: Hu
Applications: ICC/IF, IHC, IHC-P
H00142891-P01
Species: Hu
Applications: ELISA, AP, PA, WB
H00007357-M03
Species: Hu
Applications: ELISA, WB
DCP00
Species: Hu
Applications: ELISA
NB100-56583
Species: Hu, Rb, Rt
Applications: Flow, IHC, IHC-P, KO, WB
MMP200
Species: Ca, Hu, Mu, Po, Rt
Applications: ELISA
NBP1-92436
Species: Hu
Applications: WB, IHC

Publications for Sphingomyelin Synthase 2 Antibody (NBP1-92436) (0)

There are no publications for Sphingomyelin Synthase 2 Antibody (NBP1-92436).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Sphingomyelin Synthase 2 Antibody (NBP1-92436) (0)

There are no reviews for Sphingomyelin Synthase 2 Antibody (NBP1-92436). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Sphingomyelin Synthase 2 Antibody (NBP1-92436) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional Sphingomyelin Synthase 2 Products

Blogs on Sphingomyelin Synthase 2

There are no specific blogs for Sphingomyelin Synthase 2, but you can read our latest blog posts.
mFluor Violet Conjugated Antibodies

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Sphingomyelin Synthase 2 Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol SGMS2