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SPAG8 Antibody

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Immunohistochemistry-Paraffin: SPAG8 Antibody [NBP2-39092] - Staining of human testis shows high expression.
Immunohistochemistry-Paraffin: SPAG8 Antibody [NBP2-39092] - Staining of human prostate shows low expression as expected.
Orthogonal Strategies: Immunohistochemistry-Paraffin: SPAG8 Antibody [NBP2-39092] - Staining in human testis and prostate tissues using anti-SPAG8 antibody. Corresponding SPAG8 RNA-seq data are presented for the ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

Order Details

SPAG8 Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: PGSGPGHGSGSHPGPASGPGPDTGPDSELSPCIPPGFRNLVADRVPNYTSWSQHCPWEPQKQPPWEFLQVLEPGARGLWKP
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SPAG8
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Control Peptide
SPAG8 Protein (NBP2-39092PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for SPAG8 Antibody

  • BS-84
  • HSD-1MGC26201
  • hSMP-1
  • SMP1
  • SMP-1
  • SPAG3
  • sperm associated antigen 8
  • Sperm membrane protein 1
  • Sperm membrane protein BS-84
  • sperm-associated antigen 8

Background

The correlation of anti-sperm antibodies with cases of unexplained infertility implicates a role for these antibodies in blocking fertilization. Improved diagnosis and treatment of immunologic infertility, as well as identification of proteins for targeted contraception, are dependent on the identification and characterization of relevant sperm antigens. The protein encoded by this gene is recognized by sperm agglutinating antibodies from an infertile woman. This protein is localized in germ cells of the testis at all stages of spermatogenesis and is localized to the acrosomal region of mature spermatozoa. Alternatively spliced variants that encode different protein isoforms have been described but the full-length sequences of only two have been determined.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol SPAG8
Uniprot