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SP1 Antibody (4C8)

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Western Blot: SP1 Antibody (4C8) [H00006667-M03] - Analysis of SP1 expression in Hela S3 NE (Cat # L013V3).
Western Blot: SP1 Antibody (4C8) [H00006667-M03] - Analysis of SP1 expression in Hela S3 NE (Cat # L013V3).
Immunocytochemistry/ Immunofluorescence: SP1 Antibody (4C8) [H00006667-M03] - Analysis of monoclonal antibody to SP1 on HeLa cell. Antibody concentration 20 ug/ml
Immunohistochemistry-Paraffin: SP1 Antibody (4C8) [H00006667-M03] - Analysis of monoclonal antibody to SP1 on formalin-fixed paraffin-embedded human smooth muscle. Antibody concentration 3 ug/ml
Immunohistochemistry-Paraffin: SP1 Antibody (4C8) [H00006667-M03] - Analysis of monoclonal antibody to SP1 on formalin-fixed paraffin-embedded human small Intestine. Antibody concentration 3 ug/ml
Sandwich ELISA: SP1 Antibody (4C8) [H00006667-M03] - Detection limit for recombinant GST tagged SP1 is approximately 0.3ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC
Clone
4C8
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

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SP1 Antibody (4C8) Summary

Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Immunogen
SP1 (NP_612482, 89 a.a. ~ 198 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. GTGELDLTATQLSQGANGWQIISSSSGATPTSKEQSGSSTNGSNGSESSKNRTVSGGQYVVAAAPNLQNQQVLTGLPGVMPNIQYQVIPQFQTVDGQQLQFAATGAQVQQ
Specificity
SP1 (4C8)
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
SP1
Purity
IgG purified
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Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Sandwich ELISA
  • Western Blot
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF, IHC-P and ELISA.
Control
HeLa Nuclear Cell Lysate

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for SP1 Antibody (4C8)

  • Sp1 transcription factor
  • specificity protein 1
  • transcription factor Sp1
  • TSFP1

Background

Transcription factor that can activate or repress transcription in response to physiological and pathological stimuli. Binds with high affinity to GC-rich motifs and regulates the expression of a large number of genes involved in a variety of processes such as cell growth, apoptosis, differentiation and immune responses. Highly regulated by post-translational modifications (phosphorylations, sumoylation, proteolytic cleavage, glycosylation and acetylation). Binds also the PDGFR-alpha G-box promoter. May have a role in modulating the cellular response to DNA damage. Implicated in chromatin remodeling

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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H00006667-M03
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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for SP1 Antibody (H00006667-M03) (0)

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Control Lysate(s)

HeLa Nuclear Cell Lysate

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol SP1
Entrez
OMIM
Uniprot