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SOX9 Antibody (326820) [Unconjugated]

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SOX9 was detected in immersion fixed U‑251 MG human glioblastoma cells (positive) and absent in HEL 92.1.7 human erythroleukemic cells (negative) using Mouse Anti-Human SOX9 Monoclonal Antibody (Catalog # MAB3075) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
SOX9 was detected in immersion fixed U‑251 MG human glioblastoma cells (positive) and absent in HEL 92.1.7 human erythroleukemic cells (negative) using Mouse Anti-Human SOX9 Monoclonal Antibody (Catalog # ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ICC/IF
Clone
326820
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB3075-SP
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SOX9 Antibody (326820) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human SOX9
Met1-Lys151
Accession # P48436
Specificity
Detects human SOX9 in direct ELISA.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Immunocytochemistry 8-25 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SOX9 Antibody (326820) [Unconjugated]

  • campomelic dysplasia, autosomal sex-reversal
  • CMD 1
  • CMD1
  • CMPD1
  • SOX9
  • SRA1SRY (sex-determining region Y)-box 9 protein
  • SRY (sex determining region Y)-box 9
  • SRY-related HMG-box, gene 9
  • transcription factor SOX-9

Background

SOX9 belongs to the SOX (SRY-like HMG box) family of transcription factors with diverse roles in development. SOX9 is expressed in mesenchymal progenitors that give rise to chondrocytes and osteoblasts. It is also expressed in the central nervous system, neural crest, intestine, pancreas, and testis. Mutations in SOX9 are associated with defects in sex determination, cartilage and bone development, as well as abnormalities of the heart, kidneys, brain, gut, and pancreas.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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