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SOX6 Antibody (CL5690)

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Orthogonal Strategies: Immunohistochemistry-Paraffin: SOX6 Antibody (CL5690) [NBP2-61423] - Analysis in human small intestine and tonsil tissues using NBP2-61423 antibody. Corresponding SOX6 RNA-seq data are ...read more
Immunocytochemistry/ Immunofluorescence: SOX6 Antibody (CL5690) [NBP2-61423] - Staining of CACO-2 cells using the Anti-SOX6 monoclonal antibody, showing specific staining in the nucleoplasm in green. Microtubule- and ...read more
Immunohistochemistry-Paraffin: SOX6 Antibody (CL5690) [NBP2-61423] - Staining of human tonsil shows no nuclear immunoreactivity in lymphoid cells as expected (negative control).
Immunohistochemistry-Paraffin: SOX6 Antibody (CL5690) [NBP2-61423] - Staining of human glioma shows moderate to strong nuclear positivity in tumor cells.
Immunohistochemistry-Paraffin: SOX6 Antibody (CL5690) [NBP2-61423] - Staining of human testis shows moderate nuclear immunoreactivity in a subset of cells in seminiferous tubules.
Immunohistochemistry-Paraffin: SOX6 Antibody (CL5690) [NBP2-61423] - Staining of human small intestine shows moderate nuclear positivity in glandular cells.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ICC/IF, IHC
Clone
CL5690
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

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SOX6 Antibody (CL5690) Summary

Immunogen
This antibody was developed against a synthetic peptide corresponding to amino acids: NLDTFEHGGGHSYNHKQIE
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
SOX6
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 2-10 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: PFA/Triton X-100

Reactivity Notes

Mouse (84%), Rat (89%).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Protein A purified

Alternate Names for SOX6 Antibody (CL5690)

  • HSSOX6
  • SOX6
  • SOXD
  • SRY (sex determining region Y)-box 6
  • SRY-box containing gene 6
  • transcription factor SOX-6

Background

Sox genes comprise a family of genes that are related to the mammalian sex determining gene SRY. These genes similarly contain sequences that encode for the HMG-box domain, which is responsible for the sequence-specific DNA-binding activity. Sox genes encode putative transcriptional regulators implicated in the decision of cell fates during development and the control of diverse developmental processes. The highly complex group of Sox genes cluster at a minimum of 40 different loci that rapidly diverged in various animal lineages. At present 30 Sox genes have been identified, and members of this family have been shown to be conserved during evolution and to play key roles during animal development. Some are involved in human diseases, including sex reversal.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol SOX6