![Western Blot: Sodium Potassium ATPase Alpha 1 Antibody (HL114) [NBP3-13660] - A431 whole cell and membrane extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membranes were blotted with Sodium Potassium ATPase Alpha 1 antibody [HL114] (NBP3-13660) diluted at 1:500 and competitor's antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Sodium-Potassium-ATPase-Alpha-1-Antibody-HL114-Western-Blot-NBP3-13660-img0004.jpg "Western Blot: Sodium Potassium ATPase Alpha 1 Antibody (HL114) [NBP3-13660] - A431 whole cell and membrane extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membranes were blotted with Sodium Potassium ATPase Alpha 1 antibody [HL114] (NBP3-13660) diluted at 1:500 and competitor's antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clone | HL114 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Description | Centrifuge briefly prior to opening. |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | The immunogen used to generate this antibody corresponds to chicken Sodium Potassium ATPase Alpha 1. |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | ATP1A1 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | No Preservative |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for Sodium Potassium ATPase Alpha 1 Antibody (NBP3-13660)Find related products by research area.
|
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | ATP1A1 |
![Immunocytochemistry/Immunofluorescence: Sodium Potassium ATPase Alpha 1 Antibody (HL114) [NBP3-13660] - Sodium Potassium ATPase Alpha 1 antibody [HL114] detects Sodium Potassium ATPase Alpha 1 protein at cell membrane by immunofluorescent analysis. Sample: A431 cells were fixed in ice-cold MeOH for 5 min. Green: Sodium Potassium ATPase Alpha 1 stained by Sodium Potassium ATPase Alpha 1 antibody [HL114] (NBP3-13660) diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/Sodium-Potassium-ATPase-Alpha-1-Antibody-HL114-Immunocytochemistry-Immunofluorescence-NBP3-13660-img0002.jpg "Immunocytochemistry/Immunofluorescence: Sodium Potassium ATPase Alpha 1 Antibody (HL114) [NBP3-13660] - Sodium Potassium ATPase Alpha 1 antibody [HL114] detects Sodium Potassium ATPase Alpha 1 protein at cell membrane by immunofluorescent analysis. Sample: A431 cells were fixed in ice-cold MeOH for 5 min. Green: Sodium Potassium ATPase Alpha 1 stained by Sodium Potassium ATPase Alpha 1 antibody [HL114] (NBP3-13660) diluted at 1:500. Blue: Fluoroshield with DAPI.")
![Immunohistochemistry-Paraffin: Sodium Potassium ATPase Alpha 1 Antibody (HL114) [NBP3-13660] - Sodium Potassium ATPase Alpha 1 antibody [HL114] detects Sodium Potassium ATPase Alpha 1 protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. Sodium Potassium ATPase Alpha 1 stained by Sodium Potassium ATPase Alpha 1 antibody [HL114] (NBP3-13660) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/Sodium-Potassium-ATPase-Alpha-1-Antibody-HL114-Immunohistochemistry-Paraffin-NBP3-13660-img0003.jpg "Immunohistochemistry-Paraffin: Sodium Potassium ATPase Alpha 1 Antibody (HL114) [NBP3-13660] - Sodium Potassium ATPase Alpha 1 antibody [HL114] detects Sodium Potassium ATPase Alpha 1 protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. Sodium Potassium ATPase Alpha 1 stained by Sodium Potassium ATPase Alpha 1 antibody [HL114] (NBP3-13660) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Western Blot: Sodium Potassium ATPase Alpha 1 Antibody (HL114) [NBP3-13660] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Sodium Potassium ATPase Alpha 1 antibody [HL114] (NBP3-13660) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Sodium-Potassium-ATPase-Alpha-1-Antibody-HL114-Western-Blot-NBP3-13660-img0001.jpg "Western Blot: Sodium Potassium ATPase Alpha 1 Antibody (HL114) [NBP3-13660] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Sodium Potassium ATPase Alpha 1 antibody [HL114] (NBP3-13660) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: Sodium Potassium ATPase Alpha 1 Antibody (HL114) [NBP3-13660] - Sodium Potassium ATPase Alpha 1 antibody [HL114] detects Sodium Potassium ATPase Alpha 1 protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse tissues.Sodium Potassium ATPase Alpha 1 stained by Sodium Potassium ATPase Alpha 1 antibody [HL114] (NBP3-13660) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-13660_rabbit-sodium-potassium-atpase-alpha-1-mab-hl114-910202420131225.jpg "Immunohistochemistry-Paraffin: Sodium Potassium ATPase Alpha 1 Antibody (HL114) [NBP3-13660] - Sodium Potassium ATPase Alpha 1 antibody [HL114] detects Sodium Potassium ATPase Alpha 1 protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse tissues.Sodium Potassium ATPase Alpha 1 stained by Sodium Potassium ATPase Alpha 1 antibody [HL114] (NBP3-13660) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Western Blot: Sodium Potassium ATPase Alpha 1 Antibody (HL114) [NBP3-13660] - Drosophila tissue extract (5 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Sodium Potassium ATPase Alpha 1 antibody [HL114] (NBP3-13660) diluted at 1:30000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13660_rabbit-sodium-potassium-atpase-alpha-1-mab-hl114-9102024205691.jpg "Western Blot: Sodium Potassium ATPase Alpha 1 Antibody (HL114) [NBP3-13660] - Drosophila tissue extract (5 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Sodium Potassium ATPase Alpha 1 antibody [HL114] (NBP3-13660) diluted at 1:30000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Western Blot: Sodium Potassium ATPase Alpha 1 Antibody (HL114) [NBP3-13660] - A431 whole cell and membrane extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Sodium Potassium ATPase Alpha 1 antibody [HL114] (NBP3-13660) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13660_rabbit-sodium-potassium-atpase-alpha-1-mab-hl114-910202420134041.jpg "Western Blot: Sodium Potassium ATPase Alpha 1 Antibody (HL114) [NBP3-13660] - A431 whole cell and membrane extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Sodium Potassium ATPase Alpha 1 antibody [HL114] (NBP3-13660) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Western Blot RB1 Antibody (607121) [Unconjugated]](https://images.novusbio.com/images2/RB1_MAB6495_Western_Blot_10216.jpg)
![Immunocytochemistry RB1 Antibody (607121) [Unconjugated]](https://images.novusbio.com/images2/RB1_MAB6495_Immunocytochemistry_10464.jpg)
![Simple Western RB1 Antibody (607121) [Unconjugated]](https://images.novusbio.com/images2/RB1_MAB6495_Simple_Western_16629.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")