![Western Blot: SNX27 Antibody [NBP1-45282] - WB analysis of SNX27 in human liver.](http://images.novusbio.com/fullsize/SNX27-Antibody-Western-Blot-NBP1-45282-img0004.jpg "Western Blot: SNX27 Antibody [NBP1-45282] - WB analysis of SNX27 in human liver.")
| Reactivity | Hu, Mu, Rt, BvSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | Synthetic peptide made to an internal portion of human SNX27 (within residues 150-200). [Swiss-Prot# Q96L92] |
| Localization | Cytoplasm cytosol. Early endosome. Note: In T lymphocytes, recruited from the cytosol to sorting endosomes by phosphoinositide-3-kinase products. |
| Predicted Species | Rat (94%), Bovine (94%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | SNX27 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | This SNX27 antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry on paraffin-embedded sections and Western blot, where a band is seen at ~64 kDa. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Theoretical MW | 64 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Control Peptide |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS and 30% Glycerol |
| Preservative | 0.1% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Immunogen affinity purified |
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| Mouse Brain Whole Tissue Lysate (Adult Whole Normal) | |
| Human Brain Whole Tissue Lysate (Adult Whole Normal) |
Secondary Antibodies |
Isotype Controls |
Research Areas for SNX27 Antibody (NBP1-45282)Find related products by research area.
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Spotlight on SNX27 Antibodies Sorting Nexin Family Member 27 (SNX27) binds the cell membrane to regulate endocytic vesicular and protein trafficking. SNX27 has also been shown to play a key role in the endocytic recycling pathway, whereby internalized membrane proteins and lipids ... Read full blog post. |
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![Immunocytochemistry/Immunofluorescence: SNX27 Antibody [NBP1-45282] - Detection of SNX27 (Green) in Hela cells using NBP1-45282. Nuclei (Blue) are counterstained using Hoechst 33258.](http://images.novusbio.com/fullsize/SNX27-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-45282-img0006.jpg "Immunocytochemistry/Immunofluorescence: SNX27 Antibody [NBP1-45282] - Detection of SNX27 (Green) in Hela cells using NBP1-45282. Nuclei (Blue) are counterstained using Hoechst 33258.")
![Immunohistochemistry: SNX27 Antibody [NBP1-45282] - IHC analysis of SNX27 in mouse bladder using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/SNX27-Antibody-Immunohistochemistry-NBP1-45282-img0005.jpg "Immunohistochemistry: SNX27 Antibody [NBP1-45282] - IHC analysis of SNX27 in mouse bladder using DAB with hematoxylin counterstain.")
![Western Blot: Human Brain Whole Tissue Lysate (Adult Normal) [NB820-59177] - Beta Actin expression on human brain whole tissue lysate using anti-Beta Actin antibody (Cat. # NBP1-47423). Image from verified customer review.](https://images.novusbio.com/images/Human-Brain-Whole-Tissue-Lysate-Adult-Normal-Western-Blot-NB820-59177-img0001.jpg "Western Blot: Human Brain Whole Tissue Lysate (Adult Normal) [NB820-59177] - Beta Actin expression on human brain whole tissue lysate using anti-Beta Actin antibody (Cat. # NBP1-47423). Image from verified customer review.")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")