![Western Blot: SMPD1 Antibody [NBP2-22365] - Lane 1: K562 whole cell lysate Lane 2: MCF-7 whole cell lysate Lane 3: HepG2 whole cell lysate Lane 4: A431 whole cell lysate |Lysates/proteins at 20 ug per lane. Secondary: IgG (HRP) Goat Anti-Rabbit (1:10000) Predicted band size: 70kD Blocking/Dilution buffer: 5% NFDM/TBST.](http://images.novusbio.com/fullsize/SMPD1-Antibody-Western-Blot-NBP2-22365-img0004.jpg "Western Blot: SMPD1 Antibody [NBP2-22365] - Lane 1: K562 whole cell lysate Lane 2: MCF-7 whole cell lysate Lane 3: HepG2 whole cell lysate Lane 4: A431 whole cell lysate |Lysates/proteins at 20 ug per lane. Secondary: IgG (HRP) Goat Anti-Rabbit (1:10000) Predicted band size: 70kD Blocking/Dilution buffer: 5% NFDM/TBST.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, Flow, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Description | May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| Immunogen | KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 391-419 in the C-terminal region of human SMPD1 (UniProt Accession #P17405). Species sequence homology: bovine |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | SMPD1 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Application Notes | Immunohistochemistry (FFPE): Requires HIER using EDTA, pH 8.0 buffer pH9.0 prior to staining. Optimal dilutions to be determined by the researcher. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.2) |
| Preservative | 0.09% Sodium Azide |
| Purity | Protein A purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for SMPD1 Antibody (NBP2-22365)Find related products by research area.
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![Immunohistochemistry-Paraffin: SMPD1 Antibody [NBP2-22365] - Immunohistochemical analysis of paraffin-embedded human breast tissue using SMPD1 antibody (1:500) after HIER using EDTA buffer pH9.0 prior to staining performed on the Leica(R) BOND RXm. An undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody..](http://images.novusbio.com/fullsize/SMPD1-Antibody-Immunohistochemistry-Paraffin-NBP2-22365-img0006.jpg "Immunohistochemistry-Paraffin: SMPD1 Antibody [NBP2-22365] - Immunohistochemical analysis of paraffin-embedded human breast tissue using SMPD1 antibody (1:500) after HIER using EDTA buffer pH9.0 prior to staining performed on the Leica(R) BOND RXm. An undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody..")
![Flow Cytometry: SMPD1 Antibody [NBP2-22365] - Indirect Flow Cytometric analysis of K562 cells (right histogram) using SMPD1 antibody compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.](http://images.novusbio.com/fullsize/SMPD1-Antibody-Flow-Cytometry-NBP2-22365-img0007.jpg "Flow Cytometry: SMPD1 Antibody [NBP2-22365] - Indirect Flow Cytometric analysis of K562 cells (right histogram) using SMPD1 antibody compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.")
![Western Blot: SMPD1 Antibody [NBP2-22365] - Lane 1: human cerebellum lysate Lane 2: HepG2 whole cell lysate Lane 3: K562 whole cell lysate Lane 4: MCF-7 whole cell lysate Lysates/proteins at 20ug/lane. Secondary: IgG, (H+L) (HRP) goat anti-rabbit (1:10,000). Predicted band size: 70kD |Blocking/Dilution buffer: 5% NFDM/TBST.](http://images.novusbio.com/fullsize/SMPD1-Antibody-Western-Blot-NBP2-22365-img0002.jpg "Western Blot: SMPD1 Antibody [NBP2-22365] - Lane 1: human cerebellum lysate Lane 2: HepG2 whole cell lysate Lane 3: K562 whole cell lysate Lane 4: MCF-7 whole cell lysate Lysates/proteins at 20ug/lane. Secondary: IgG, (H+L) (HRP) goat anti-rabbit (1:10,000). Predicted band size: 70kD |Blocking/Dilution buffer: 5% NFDM/TBST.")
![Immunohistochemistry-Paraffin: SMPD1 Antibody [NBP2-22365] - Immunohistochemistry analysis of formalin fixed paraffin embedded human testis tissue using SMPD1 antibody (1:500) after HIER using EDTA buffer pH9.0 prior to staining performed on the Leica(R) BOND RXm. An undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody](http://images.novusbio.com/fullsize/SMPD1-Antibody-Immunohistochemistry-Paraffin-NBP2-22365-img0005.jpg "Immunohistochemistry-Paraffin: SMPD1 Antibody [NBP2-22365] - Immunohistochemistry analysis of formalin fixed paraffin embedded human testis tissue using SMPD1 antibody (1:500) after HIER using EDTA buffer pH9.0 prior to staining performed on the Leica(R) BOND RXm. An undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody")
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