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Recombinant Human Smad2 His Protein

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SDS-Page: Recombinant Human Smad2 Protein [NBC1-25856] - SMAD2, 54.4 kDa (487aa) with a purity of 95% by SDS - PAGE
SDS-Page: Recombinant Human Smad2 Protein [NBC1-25856] - SMAD2, 54.4 kDa (487aa) with a purity of 95% by SDS - PAGE

Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE
Concentration
0.5 mg/ml

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Recombinant Human Smad2 His Protein Summary

Description
A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1- 467 of Human Smad2

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MSSILPFTPP VVKRLLGWKK SAGGSGGAGG GEQNGQEEKW CEKAVKSLVK KLKKTGRLDE LEKAITTQNC NTKCVTIPST CSEIWGLSTP NTIDQWDTTG LYSFSEQTRS LDGRLQVSHR KGLPHVIYCR LWRWPDLHSH HELKAIENCE YAFNLKKDEV CVNPYHYQRV ETPVLPPVLV PRHTEILTEL PPLDDYTHSI PENTNFPAGI EPQSNYIPET PPPGYISEDG ETSDQQLNQS MDTGSPAELS PTTLSPVNHS LDLQPVTYSE PAFWCSIAYY ELNQRVGETF HASQPSLTVD GFTDPSNSER FCLGLLSNVN RNATVEMTRR HIGRGVRLYY IGGEVFAECL SDSAIFVQSP NCNQRYGWHP ATVCKIPPGC NLKIFNNQEF AALLAQSVNQ GFEAVYQLTR MCTIRMSFVK GWGAEYRRQT VTSTPCWIEL HLNGPLQWLD KVLTQMGSPS VRCSSMS
Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
SMAD2
Purity
>90%, by SDS-PAGE
Endotoxin Note
< 1.0 EU per 1 microgram of protein (determined by LAL method)

Applications/Dilutions

Dilutions
  • SDS-Page
Theoretical MW
54.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NBC1-25856 in the following application:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20 mM Tris-HCl buffer (pH8.0), 0.1 M NaCl, 20% glycerol
Preservative
No Preservative
Concentration
0.5 mg/ml
Purity
>90%, by SDS-PAGE

Alternate Names for Recombinant Human Smad2 His Protein

  • hMAD-2
  • hSMAD2
  • JV18
  • JV18-1
  • JV18-1SMAD, mothers against DPP homolog 2 (Drosophila)
  • MAD homolog 2
  • Mad protein homolog
  • Mad, mothers against decapentaplegic homolog 2
  • MADH2
  • MADH2MGC34440
  • MADR2
  • MADR2MGC22139
  • Mad-related protein 2
  • mother against DPP homolog 2
  • mothers against decapentaplegic homolog 2
  • Mothers against DPP homolog 2
  • Sma- and Mad-related protein 2
  • SMAD 2
  • SMAD family member 2MAD, mothers against decapentaplegic homolog 2 (Drosophila)
  • SMAD, mothers against DPP homolog 2
  • Smad2
  • Smox

Background

SMAD2(Mothers against decapentaplegic homolog 2) belongs to the SMAD family of proteins that mediate signal transduction by the TGF-beta/activin/BMP-2/4 cytokine superfamily from receptor Ser/Thr protein kinases at the cell surface to the nucleus. Phosphorylated Smad2 is then able to form a complex with Smad4 or SARA. These complexes accumulate in the cell nucleus, where they are directly participating in the regulation of gene expression. Recombinant SMAD2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Recombinant Human
Smad2 His Protein
NBC1-25856
Recombinant Human Smad2 His Protein
Species: Hu
Applications: PAGE

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Review for Smad2 Recombinant Protein (NBC1-25856) (1) 51

Average Rating: 5
(Based on 1 review)

Reviews using NBC1-25856:
Filter by Applications
Immunoassay Standard
(1)
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  5
reviewed by:
Verified Customer
 Immunoassay Standard 10/05/2020
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Summary

ApplicationImmunoassay Standard
Lot1732101
CommentsProtein served as a perfect standard for our MSD Assay

FAQs for Smad2 Recombinant Protein (NBC1-25856) (0)

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Recent Reviews

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 Verified Customer
10/05/2020
Application: Immunoassay Standard
Species:
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Bioinformatics

Gene Symbol SMAD2