![Western Blot: Sirtuin 1/SIRT1 Antibody (1F3) - Azide and BSA Free [NBP2-80949] - Western blot analysis using SIRT1 mouse mAb against MCF-7 (1), Jurkat (2), Hela (3), HEK293 (4) and A549 (5) cell lysates. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/Sirtuin-1-SIRT1-Antibody-1F3-Azide-and-BSA-Free-Western-Blot-NBP2-80949-img0001.jpg "Western Blot: Sirtuin 1/SIRT1 Antibody (1F3) - Azide and BSA Free [NBP2-80949] - Western blot analysis using SIRT1 mouse mAb against MCF-7 (1), Jurkat (2), Hela (3), HEK293 (4) and A549 (5) cell lysates. Image from the standard format of this antibody.")
| Reactivity | Hu, Mu, Rt, Pm, RbSpecies Glossary |
| Applications | WB, ELISA, Flow, ICC/IF, IHC, CyTOF-ready, IHC |
| Clone | 1F3 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | Purified recombinant fragment of human SIRT1 (amino acids: 265-452) expressed in E. coli. [UniProt# Q96EB6] |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | SIRT1 |
| Purity | Ammonium sulfate precipitation |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 120 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | No Preservative |
| Concentration | 1 mg/ml |
| Purity | Ammonium sulfate precipitation |
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Secondary Antibodies |
Isotype Controls |
Research Areas for Sirtuin 1/SIRT1 Antibody (NBP2-80949)Find related products by research area.
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| Gene Symbol | SIRT1 |
![Immunocytochemistry/Immunofluorescence: Sirtuin 1/SIRT1 Antibody (1F3) - Azide and BSA Free [NBP2-80949] - SIRT1 antibody was tested at 1:10 in Ntera2 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). Image objective 40x. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/Sirtuin-1-SIRT1-Antibody-1F3-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-80949-img0003.jpg "Immunocytochemistry/Immunofluorescence: Sirtuin 1/SIRT1 Antibody (1F3) - Azide and BSA Free [NBP2-80949] - SIRT1 antibody was tested at 1:10 in Ntera2 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). Image objective 40x. Image from the standard format of this antibody.")
![Immunohistochemistry: Sirtuin 1/SIRT1 Antibody (1F3) - Azide and BSA Free [NBP2-80949] - Immunohistochemical analysis of paraffin-embedded lung cancer tissues (left) and kidney cancer tissues (right) using SIRT1 mouse mAb with DAB staining. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/Sirtuin-1-SIRT1-Antibody-1F3-Azide-and-BSA-Free-Immunohistochemistry-NBP2-80949-img0004.jpg "Immunohistochemistry: Sirtuin 1/SIRT1 Antibody (1F3) - Azide and BSA Free [NBP2-80949] - Immunohistochemical analysis of paraffin-embedded lung cancer tissues (left) and kidney cancer tissues (right) using SIRT1 mouse mAb with DAB staining. Image from the standard format of this antibody.")
![Flow Cytometry: Sirtuin 1/SIRT1 Antibody (1F3) - Azide and BSA Free [NBP2-80949] - Intracellular flow cytometric staining of 1 x 10^6 HEK-293 cells using SIRT1 antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/Sirtuin-1-SIRT1-Antibody-1F3-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80949-img0006.jpg "Flow Cytometry: Sirtuin 1/SIRT1 Antibody (1F3) - Azide and BSA Free [NBP2-80949] - Intracellular flow cytometric staining of 1 x 10^6 HEK-293 cells using SIRT1 antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used. Image from the standard format of this antibody.")
![Immunocytochemistry/Immunofluorescence: Sirtuin 1/SIRT1 Antibody (1F3) - Azide and BSA Free [NBP2-80949] - Analysis of NTERA-2 cells using SIRT1 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/Sirtuin-1-SIRT1-Antibody-1F3-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-80949-img0002.jpg "Immunocytochemistry/Immunofluorescence: Sirtuin 1/SIRT1 Antibody (1F3) - Azide and BSA Free [NBP2-80949] - Analysis of NTERA-2 cells using SIRT1 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Image from the standard format of this antibody.")
![Flow Cytometry: Sirtuin 1/SIRT1 Antibody (1F3) - Azide and BSA Free [NBP2-80949] - Flow cytometric analysis of K562 cells using SIRT1 mouse mAb (green) and negative control (purple). Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/Sirtuin-1-SIRT1-Antibody-1F3-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80949-img0005.jpg "Flow Cytometry: Sirtuin 1/SIRT1 Antibody (1F3) - Azide and BSA Free [NBP2-80949] - Flow cytometric analysis of K562 cells using SIRT1 mouse mAb (green) and negative control (purple). Image from the standard format of this antibody.")
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![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")