| Reactivity | HuSpecies Glossary |
| Applications | Flow, B/N, CyTOF-ready, ELISA(Cap) |
| Clone | 194128 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | LYOPH |
| Immunogen | Mouse myeloma cell line NS0-derived recombinant human Siglec‑5/Siglec‑14 Lys18-Thr434 Accession # O15389 |
| Specificity | Detects human Siglec‑5/Siglec‑14 in ELISAs. In sandwich immunoassays, 100% cross-reactivity with recombinant human (rh) Siglec-14 is observed and no cross-reactivity with rhSiglec-3, rhSiglec-7, or rhSiglec-9 is observed. |
| Source | N/A |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | SIGLEC5 |
| Purity Statement | Protein A or G purified from hybridoma culture supernatant |
| Endotoxin Note | <0.10 EU per 1 μg of the antibody by the LAL method. |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
|
| Application Notes | ELISA Capture: Human Siglec-5/Siglec-14 Antibody (Catalog # MAB10721) ELISA Detection: Human Siglec-5/Siglec-14 Biotinylated Antibody (Catalog # BAM10722) Standard: Recombinant Human Siglec-5/CD170 Fc Chimera (Catalog # 1072-SL) |
|
| Publications |
|
| Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
| Buffer | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS. |
| Preservative | No Preservative |
| Concentration | LYOPH |
| Reconstitution Instructions | Reconstitute at 0.5 mg/mL in sterile PBS. |
Siglecs (sialic acid binding Ig-like lectins) are a subgroup of the immunoglobulin superfamily that interact with sialic acids in glycoproteins and glycolipids. Siglec-5 binds to alpha-2, 3- and alpha-2, 6-linked sialic acid equally. It occurs as a disulfide-linked dimer of approximately 140 kDa with the highest expression levels in hematopoietic tissues. Siglec-5 has an inhibitory motif within its cytoplasmic domain. Siglec-14 is an activating receptor that shares 99.5% aa sequence identity with Siglec-5 through the first two extracellular Ig domains and displays a similar glycan binding preference.
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![Bioactivity CTLA-4 [Unconjugated]](https://images.novusbio.com/images2/CTLA4_7268CT_2293.jpg)
![Flow Cytometry Siglec-8 Antibody (837535) [Unconjugated]](https://images.novusbio.com/images2/Siglec8_MAB7975_Flow_Cytometry_13051.jpg)
![Neutralization Siglec-7/CD328 Antibody [Unconjugated]](https://images.novusbio.com/images2/Siglec-7_AF1138_Block_Neutralize_8760.jpg)
![SDS-PAGE TNF-alpha [Unconjugated]](https://images.novusbio.com/images2/TNF-alpha_210-TA_256.jpg)
![Bioactivity TNF-alpha [Unconjugated]](https://images.novusbio.com/images2/TNFalpha_210TA_1658.jpg)
![SEC-MALS TNF-alpha [Unconjugated]](https://images.novusbio.com/images/210-ta_recombinant-human-tnf-alpha-protein-sec-mals-35202312244..jpg)
![Neutralization Siglec-F Antibody (238047) [Unconjugated]](https://images.novusbio.com/images2/Siglec-F_MAB17061_Block_Neutralize_9039.jpg)
![Flow Cytometry Siglec-6/CD327 Antibody (767329) [Unconjugated]](https://images.novusbio.com/images2/Siglec6_MAB2859_Flow_Cytometry_15540.jpg)
![Flow Cytometry Siglec-6/CD327 Antibody (767329) [Unconjugated]](https://images.novusbio.com/images2/Siglec6_MAB2859_Flow_Cytometry_14724.jpg)
![Flow Cytometry CXCR5 Antibody (51505) [Unconjugated]](https://images.novusbio.com/images2/CXCR5_MAB190_Flow_Cytometry_22006.jpg)
![Immunohistochemistry CXCR5 Antibody (51505) [Unconjugated]](https://images.novusbio.com/images2/CXCR5_MAB190_Flow_Cytometry_18756.jpg)
![Neutralization CXCR5 Antibody (51505) [Unconjugated]](https://images.novusbio.com/images2/CXCR5_MAB190_Block_Neutralize_9046.jpg)
![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")