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BTLA/CD272 Antibody (OTI2E4)

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Western Blot: BTLA/CD272 Antibody (2E4) [NBP2-45549] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BTLA.
Immunohistochemistry: BTLA/CD272 Antibody (2E4) [NBP2-45549] - Analysis of Carcinoma of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Flow Cytometry: BTLA/CD272 Antibody (2E4) [NBP2-45549] - Analysis of live Sup-B15 cells, using BTLA antibody , compared to IgG1 isotype control (Black).
Immunohistochemistry: BTLA/CD272 Antibody (2E4) [NBP2-45549] - Analysis of Carcinoma of Human thyroid tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: BTLA/CD272 Antibody (2E4) [NBP2-45549] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: BTLA/CD272 Antibody (2E4) [NBP2-45549] - Analysis of Human lymph node tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: BTLA/CD272 Antibody (2E4) [NBP2-45549] - Analysis of Human colon tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: BTLA/CD272 Antibody (2E4) [NBP2-45549] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: BTLA/CD272 Antibody (2E4) [NBP2-45549] - Analysis of Carcinoma of Human kidney tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: BTLA/CD272 Antibody (2E4) [NBP2-45549] - Analysis of Carcinoma of Human liver tissue.(Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: BTLA/CD272 Antibody (2E4) [NBP2-45549] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: BTLA/CD272 Antibody (2E4) [NBP2-45549] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: BTLA/CD272 Antibody (2E4) [NBP2-45549] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: BTLA/CD272 Antibody (2E4) [NBP2-45549] - Analysis of Carcinoma of Human lung tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: BTLA/CD272 Antibody (2E4) [NBP2-45549] - Analysis of Human breast tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, IHC
Clone
OTI2E4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

BTLA/CD272 Antibody (OTI2E4) Summary

Immunogen
Full length human recombinant protein of human BTLA(NP_861445) produced in HEK293T cell.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
BTLA
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:4000
Theoretical MW
32.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 3
using
NBP2-45549 in the following applications:

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for BTLA/CD272 Antibody (OTI2E4)

  • B and T lymphocyte associated
  • B and T lymphocyte attenuator
  • B- and T-lymphocyte attenuator
  • B- and T-lymphocyte-associated protein
  • BTLA
  • BTLA1
  • CD272 antigen
  • CD272
  • FLJ16065
  • MGC129743

Background

BTLA expression is induced during activation of T cells, and BTLA remains expressed on Th1 cells but not Th2 cells. Like PD1 (MIM 600244) and CTLA4 (MIM 123890), BTLA interacts with a B7 homolog, B7H4.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for BTLA/CD272 Antibody (NBP2-45549) (0)

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Review for BTLA/CD272 Antibody (NBP2-45549) (1) 31

Average Rating: 3
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP2-45549:
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ELISA
(1)
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Images Ratings Applications Species Date Details
  3
reviewed by:
Verified Customer
ELISA Human 06/21/2017
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Summary

ApplicationELISA
Sample TestedHuman serum
SpeciesHuman
LotW001

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FAQs for BTLA/CD272 Antibody (NBP2-45549) (0)

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Secondary Antibodies

 

Isotype Controls

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Verified Customer
06/21/2017
Application: ELISA
Species: Human

Bioinformatics

Gene Symbol BTLA