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Siglec-8 Antibody (837535) [Unconjugated]

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 (1 Review)
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Human peripheral blood granulocytes were stained with Rat Anti-Human CCR3 PE-conjugated Monoclonal Antibody (Catalog # FAB155P) and either (A) Mouse Anti-Human Siglec-8 Monoclonal Antibody (Catalog # MAB7975) or (B) Mouse IgG1 Isotype Control (Catalog # MAB002) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).
Human peripheral blood granulocytes were stained with Rat Anti-Human CCR3 PE-conjugated Monoclonal Antibody (Catalog # FAB155P) and either (A) Mouse Anti-Human Siglec-8 Monoclonal Antibody (Catalog # MAB7975) or (B) ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, CyTOF-ready
Clone
837535
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
FAB7975U-100UG
Alexa Fluor 405
FAB7975V-100UG
Alexa Fluor 488
FAB7975G
Alexa Fluor 532
FAB7975X
Alexa Fluor 594
FAB7975T-100UG
Alexa Fluor 647
FAB7975R-100UG
Alexa Fluor 700
FAB7975N
Alexa Fluor 750
FAB7975S-100UG
Allophycocyanin
FAB7975A
Allophycocyanin/Cy7
FAB7975APCCY7
Biotin
FAB7975B
CoraFluor 1
FAB7975CL1
DyLight 350
FAB7975D
DyLight 405
FAB7975E
DyLight 488
FAB7975K
DyLight 550
FAB7975L
DyLight 594
FAB7975M
DyLight 650
FAB7975W
DyLight 680
FAB7975Y
DyLight 755
FAB7975Z
FITC
FAB7975F
HRP
FAB7975H
Janelia Fluor 525
FAB7975JF525
Janelia Fluor 549
FAB7975I
Janelia Fluor 646
FAB7975J
Janelia Fluor 669
FAB7975JF669
PE/Atto594
FAB7975PEATT594
PE/Cy5.5
FAB7975PECY55
PE/Cy7
FAB7975PECY7
PerCP (Peridinin-chlorophyll Protein Complex)
FAB7975C
Phycoerythrin
FAB7975P
mFluor Violet 450 SE
FAB7975MFV450
mFluor Violet 500 SE
FAB7975MFV500
mFluor Violet 610 SE
FAB7975MFV610
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
FAB7975C
Carrier Free
FAB7975P
Carrier Free
FAB7975A
Carrier Free
FAB7975G
Carrier Free
FAB7975N
Carrier Free
MAB7975-SP
Carrier Free
FAB7975V-100UG
Carrier Free
FAB7975U-100UG
Carrier Free
FAB7975T-100UG
Carrier Free
FAB7975S-100UG
Carrier Free
FAB7975R-100UG
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Siglec-8 Antibody (837535) [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human Siglec-8
Met17-Ala363
Accession # Q9NYZ4
Specificity
Detects human Siglec-8 in ELISAs. In direct ELISAs, no cross-reactivity with recombinant human Siglec-7, -9, or -14 is observed.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
SIGLEC8
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
Reviewed Applications
Read 1 Review rated 4
using
MAB7975 in the following applications:
Publications
Read Publications using
MAB7975 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Siglec-8 Antibody (837535) [Unconjugated]

  • CD329 antigen
  • CDw329
  • MGC59785
  • SAF2
  • SAF2SAF-2
  • sialic acid binding Ig-like lectin 8
  • Sialoadhesin family member 2
  • Siglec8
  • Siglec-8
  • SIGLEC8L
  • SIGLEC8Lsialic acid-binding Ig-like lectin 8

Background

Siglec-8 (Sialic acid-binding Ig-like lectin 8), formerly called SAF-2 (sialoadhesin family member 2) and designated CD329, is a 75 kDa member of the inhibitory
CD33‑related SIGLEC family within the Ig superfamily of proteins. An alternately spliced 48 kDa short form lacks most of the intracellular domain, while another isoform lacks the second of three extracellular Ig domains, representing amino acids (aa) 152-245 of the 499 aa long form. Siglec-8 is expressed exclusively on eosinophils, mast cells, and weakly on basophils, and is a marker for eosinophil terminal differentiation. It specifically recognizes 6’ sulfated sialyl Lewis X carbohydrate structures. Engagement results in eosinophils apoptosis, or mast cell inhibition of degranulation. Eosinophil Siglec F is the mouse paralog of human Siglec-8, but shares <40% amino acid sequence identity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Siglec-8 Antibody (MAB7975)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: ELISA Development.


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ELISA Development
(1)
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Filter By Species
Human
(1)
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Review for Siglec-8 Antibody (MAB7975) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using MAB7975:
Filter by Applications
IHC-Fr
(1)
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Filter by Species
Human
(1)
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Images Ratings Applications Species Date Details
Immunohistochemistry-Frozen Siglec-8 MAB7975
Enlarge 		4
reviewed by:
Verified Customer
 IHC-Fr Human 03/30/2020
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Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedTonsil
SpeciesHuman
LotN/A

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FAQs for Siglec-8 Antibody (MAB7975) (0)

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Secondary Antibodies

 

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Immunohistochemistry-Frozen Siglec-8 MAB7975
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 Verified Customer
03/30/2020
Application: IHC-Fr
Species: Human
read this review

Bioinformatics

Gene Symbol SIGLEC8
Uniprot