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SHP-2/PTPN11 Antibody

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Western Blot: SHP-2/PTPN11 Antibody [NB100-806] - Human Muscle lysate (RIPA buffer, 35 ug total protein per lane). Antibody at 2 ug/mL. Detected by chemiluminescence.
Western Blot: SHP-2/PTPN11 Antibody [NB100-806] - Human Muscle lysate (RIPA buffer, 35 ug total protein per lane). Antibody at 2 ug/mL. Detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence: SHP-2/PTPN11 Antibody [NB100-806] - Analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 ...read more
Flow Cytometry: SHP-2/PTPN11 Antibody [NB100-806] - Analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, BvSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

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SHP-2/PTPN11 Antibody Summary

Immunogen
Peptide with sequence C-YENVGLMQQQKSFR corresponding to C-Terminus according to NP_002825.3.
Predicted Species
Mouse (100%), Rat (100%), Porcine (100%), Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
PTPN11
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Flow Cytometry 10 ug/mL
  • Immunocytochemistry/ Immunofluorescence 10 ug/mL
  • Peptide ELISA Detection limit 1:28000
  • Western Blot 1 - 3 ug/mL
Application Notes
WB: Approx. 70 kDa band observed in human skeletal muscle lysate (calculated MW of 68.0 kDa band according to NP_002825.3).
Control
293 Whole Cell Lysate
Human Skeletal Muscle Whole Tissue Lysate (Adult Whole Normal)
Publications
Read Publication using NB100-806.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for SHP-2/PTPN11 Antibody

  • BPTP3
  • EC 3.1.3.48
  • MGC14433
  • protein tyrosine phosphatase, non-receptor type 11
  • protein tyrosine phosphatase-2
  • Protein-tyrosine phosphatase 1D
  • Protein-tyrosine phosphatase 2C
  • PTP1D
  • PTP-1D
  • PTP2C
  • PTP-2C
  • PTP2CNS1
  • PTPN11
  • SHP2
  • SHP-2
  • SHP2CFC
  • SHPTP2
  • SH-PTP2
  • SH-PTP2Noonan syndrome 1
  • SH-PTP3
  • tyrosine-protein phosphatase non-receptor type 11

Background

A protein tyrosine phosphatase that is widely expressed and plays a regulatory role in various cell signaling events that are important for many cell functions, such as mitogenic activation, metabolic control, transcription regulation, and cell migration.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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SHP-2/PTPN11
Antibody
NB100-806
SHP-2/PTPN11 Antibody
Species: Hu, Mu, Rt, Po, Bv
Applications: WB, ELISA, Flow, ICC/IF

Publications for SHP-2/PTPN11 Antibody (NB100-806)(1)


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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for SHP-2/PTPN11 Antibody (NB100-806) (0)

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Control Lysate(s)

293 Whole Cell Lysate
Human Skeletal Muscle Whole Tissue Lysate (Adult Whole Normal)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol PTPN11
Entrez
Uniprot