![Western Blot: SHP-1 [p Ser591] Antibody [NBP3-23399] - Western blot analysis of human Jurkat cells treated with pervanadate (1 mM) for 30 min. The blot was exposed to lambda phosphatase (lanes 2 & 4) then probed with anti-SHP-1 (C-terminal) antibody (lanes 1 & 2) or anti-SHP-1 (Ser-591) antibody (lanes 3-6). The SHP-1 (Ser-591) antibody was used in the presence of phospho-SHP-1 (Ser-591) peptide (lane 5) or a non-specific phospho-serine peptide (lane 6).](http://images.novusbio.com/fullsize/nbp3-23399_rabbit-shp-1-p-ser591-pab-19920231911473.jpg "Western Blot: SHP-1 [p Ser591] Antibody [NBP3-23399] - Western blot analysis of human Jurkat cells treated with pervanadate (1 mM) for 30 min. The blot was exposed to lambda phosphatase (lanes 2 & 4) then probed with anti-SHP-1 (C-terminal) antibody (lanes 1 & 2) or anti-SHP-1 (Ser-591) antibody (lanes 3-6). The SHP-1 (Ser-591) antibody was used in the presence of phospho-SHP-1 (Ser-591) peptide (lane 5) or a non-specific phospho-serine peptide (lane 6).")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Description | Stable for at least 1 year at -20°C. |
| Immunogen | Phospho-SHP-1 (Ser-591) synthetic peptide (coupled to carrier protein) corresponding to amino acids around serine 591 in human SHP-1. The sequence is highly conserved in rat and mouse SHP-1. [UniProt# P29350] |
| Modification | p Ser591 |
| Specificity | This antibody was cross-adsorbed to a non-specific phospho-serine peptide then affinity-purified using phospho-SHP-1 (Ser-591) peptide. The antibody detects a 68 kDa band on SDS-PAGE immunoblots of human Jurkat and A431 cells treated with pervanadate, as well as A431 cells treated with EGF. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | PTPN6 |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 68 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20C. |
| Buffer | PBS, 1 mg/ml BSA and 50% glycerol |
| Preservative | 0.05% Sodium Azide |
| Purity | Antigen Affinity-purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for SHP-1 Antibody (NBP3-23399)Find related products by research area.
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | PTPN6 |
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
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