![Immunocytochemistry/Immunofluorescence: SH3BP1 Antibody [NBP1-88827] - Immunofluorescent staining of human cell line A-431 shows localization to cytosol.](http://images.novusbio.com/fullsize/SH3BP1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-88827-img0005.jpg "Immunocytochemistry/Immunofluorescence: SH3BP1 Antibody [NBP1-88827] - Immunofluorescent staining of human cell line A-431 shows localization to cytosol.")
| Reactivity | HuSpecies Glossary |
| Applications | ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | This antibody was developed against Recombinant Protein corresponding to amino acids: ENLSNLRYLMKFLARLAEEQEVNKMTPSNIAIVLGPNLLWPPEKEGDQAQLDAASVSSIQVVGVVEALIQSADTLFPGDINFNVSGLFSAVTLQDTVSDRLASEELPSTAVPTPATTPAPAPAPAPAPAPALASAATKERTESEVPP |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | SH3BP1 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
||
| Application Notes | For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100. |
||
| Control Peptide |
|
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.2) and 40% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Purity | Immunogen affinity purified |
![Western Blot AKT [p Ser473] Antibody [Unconjugated] - Pan Specific](https://images.novusbio.com/images2/Akt3_AF887_Western_Blot_5930.jpg)
![Simple Western AKT [p Ser473] Antibody [Unconjugated] - Pan Specific](https://images.novusbio.com/images2/16332.jpg)
![Intracellular Staining by Flow Cytometry AKT [p Ser473] Antibody [Unconjugated] - Pan Specific](https://images.novusbio.com/images2/Akt3_AF887_Flow_Cytometry_8283.jpg)
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | SH3BP1 |
![Immunohistochemistry-Paraffin: SH3BP1 Antibody [NBP1-88827] - Staining in human spleen and kidney tissues using anti-SH3BP1 antibody. Corresponding SH3BP1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/SH3BP1-Antibody-Immunohistochemistry-Paraffin-NBP1-88827-img0008.jpg "Immunohistochemistry-Paraffin: SH3BP1 Antibody [NBP1-88827] - Staining in human spleen and kidney tissues using anti-SH3BP1 antibody. Corresponding SH3BP1 RNA-seq data are presented for the same tissues.")
![Immunohistochemistry-Paraffin: SH3BP1 Antibody [NBP1-88827] - Staining of human colon shows cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/SH3BP1-Antibody-Immunohistochemistry-Paraffin-NBP1-88827-img0002.jpg "Immunohistochemistry-Paraffin: SH3BP1 Antibody [NBP1-88827] - Staining of human colon shows cytoplasmic positivity in glandular cells.")
![Immunohistochemistry-Paraffin: SH3BP1 Antibody [NBP1-88827] - Staining of human kidney shows low expression as expected.](http://images.novusbio.com/fullsize/SH3BP1-Antibody-Immunohistochemistry-Paraffin-NBP1-88827-img0006.jpg "Immunohistochemistry-Paraffin: SH3BP1 Antibody [NBP1-88827] - Staining of human kidney shows low expression as expected.")
![Immunohistochemistry-Paraffin: SH3BP1 Antibody [NBP1-88827] - Staining of human spleen shows high expression.](http://images.novusbio.com/fullsize/SH3BP1-Antibody-Immunohistochemistry-Paraffin-NBP1-88827-img0007.jpg "Immunohistochemistry-Paraffin: SH3BP1 Antibody [NBP1-88827] - Staining of human spleen shows high expression.")
![Western Blot c-Abl Antibody [Unconjugated]](https://images.novusbio.com/images2/c-Abl_AF5414_Western_Blot_5714.jpg)
![Simple Western c-Abl Antibody [Unconjugated]](https://images.novusbio.com/images2/cAbl_AF5414_Simple_Western_17046.jpg)
![Bioactivity Semaphorin 3A [Unconjugated]](https://images.novusbio.com/images2/Semaphorin_3A_1250S3_1473.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")