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SGLT1/SLC5A1 Antibody

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Immunohistochemistry-Paraffin: SGLT1/SLC5A1 Antibody [NBP2-27569] - Staining of paraffin embedded Human Small Intestine. Antibody at 3.8 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunohistochemistry-Paraffin: SGLT1/SLC5A1 Antibody [NBP2-27569] - Staining of paraffin embedded Human Small Intestine. Antibody at 3.8 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

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SGLT1/SLC5A1 Antibody Summary

Immunogen
Peptide with sequence C-KETIEIETQVPEKKK, from the internal region of the protein sequence according to NP_000334.1.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
SLC5A1
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 2 - 4 ug/mL
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 3 - 6 ug/mL
  • Peptide ELISA 1:4000
  • Western Blot 0.3-1 ug/ml
Application Notes
WB: Approx 75kDa band observed in isolated Brush Border membranes from Human Kidney (calculated MW of 73.5kDa according to NP_000334.1). Primary incubation was 1 hour.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for SGLT1/SLC5A1 Antibody

  • D22S675
  • High affinity sodium-glucose cotransporter
  • Na+/glucose cotransporter 1
  • NAGT
  • NAGTsodium/glucose cotransporter 1
  • SGLT1
  • SGLT1Na(+)/glucose cotransporter 1
  • SLC5A1
  • solute carrier family 5 (sodium/glucose cotransporter), member 1
  • Solute carrier family 5 member 1

Background

Glucose transporters are integral membrane proteins that mediate the transport of glucose and structurally related substances across cellular membranes. Two families of glucose transporter have been identified: the facilitated diffusion glucose transporter family (GLUT family), also known as 'uniporters,' and the sodium dependent glucose transporter family (SGLT family), also known as 'cotransporters' or 'symporters'. The SGLT1 gene encodes a protein that is involved in the active transport of glucose and galactose into eukaryotic and some prokaryotic cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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SGLT1/SLC5A1
Antibody
NBP2-27569
SGLT1/SLC5A1 Antibody
Species: Hu, Mu
Applications: WB, ELISA, ICC/IF, IHC

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Product General Protocols

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Video Protocols

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Bioinformatics

Gene Symbol SLC5A1
Uniprot