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Serum Response Factor SRF Antibody (PCRP-SRF-1F7) [Janelia Fluor® 525]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, IP, MA
Clone
PCRP-SRF-1F7
Clonality
Monoclonal
Host
Mouse
Conjugate
Janelia Fluor 525

Order Details

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Serum Response Factor SRF Antibody (PCRP-SRF-1F7) [Janelia Fluor® 525] Summary

Immunogen
Recombinant full-length human Serum Response Factor SRF protein (Uniprot: P11831)
Localization
Nucleus, Nucleoplasm
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
SRF
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Protein Array
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Notes



Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for Serum Response Factor SRF Antibody (PCRP-SRF-1F7) [Janelia Fluor® 525]

  • MCM1
  • serum response factor (c-fos serum response element-binding transcriptionfactor)
  • Serum Response Factor SRF
  • serum response factor
  • SRF

Background

Serum response factor (SRF) is a transcription factor that binds the serum response element (SRE), a sequence that mediates the transient response of many cellular genes to growth stimulation. SRF is a cardiac-enriched transcription factor that is required for the appearance of beating sarcomeres in the heart. In addition, SRF may also direct the expression of microRNAs that inhibit the expression of cardiac regulatory factors. SRF binding sites are also constitutive promotor elements in many muscle specific promoters. At the c-Fos SRE, formation of a ternary complex containing SRF and its accessory protein p62TCF appears to be important for signal transduction. Two related Ets domain proteins, Elk 1 and SRF accessory protein 1 (SAP 1) have DNA binding properties identical to that of p62TCF

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Serum Response Factor SRF Antibody (NBP3-14188JF525) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol SRF