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Serpin E1/PAI-1 Antibody (1D5) - BSA Free

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Western Blot: Serpin E1/PAI-1 Antibody (1D5) [NBP2-37532] - Analysis using SERPINE1 mAb against HEK293 (1) and SERPINE1 (AA: 194-316)-hIgGFc transfected HEK293 (2) cell lysate.
Immunohistochemistry-Paraffin: Serpin E1/PAI-1 Antibody (1D5) [NBP2-37532] - Analysis of kidney cancer tissues using SERPINE1 mouse mAb with DAB staining.
Flow Cytometry: Serpin E1/PAI-1 Antibody (1D5) [NBP2-37532] - Analysis of NIH/3T3 cells using SERPINE1 mouse mAb (green) and negative control (red).
Western Blot: Serpin E1/PAI-1 Antibody (1D5) [NBP2-37532] - Western blot analysis using SERPINE1 mAb against human SERPINE1 (AA: 194-316) recombinant protein. (Expected MW is 45kDa kDa)
Immunohistochemistry-Paraffin: Serpin E1/PAI-1 Antibody (1D5) [NBP2-37532] - Analysis of lung cancer tissues using SERPINE1 mouse mAb with DAB staining.
ELISA: Serpin E1/PAI-1 Antibody (1D5) [NBP2-37532] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, IHC, CyTOF-ready
Clone
1D5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

Order Details

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Catalog# & Formulation Size Price

Serpin E1/PAI-1 Antibody (1D5) - BSA Free Summary

Immunogen
Purified recombinant fragment of human Serpin E1/PAI-1 expressed in E. Coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
SERPINE1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • ELISA 1:10000
  • Flow Cytometry 1:200 - 1:400
  • Immunohistochemistry 1:200 - 1:1000
  • Immunohistochemistry-Paraffin 1:200 - 1:1000
  • Western Blot 1:500 - 1:2000
Application Notes
This antibody is Cytof ready.
Theoretical MW
45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using NBP2-37532.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for Serpin E1/PAI-1 Antibody (1D5) - BSA Free

  • Endothelial plasminogen activator inhibitor
  • Nexin
  • PAI1
  • PAI-1
  • PAI1PAI-1
  • PAISerpin E1
  • PLANH1
  • PLANH1plasminogen activator inhibitor 1
  • serine (or cysteine) proteinase inhibitor, clade E (nexin, plasminogenactivator inhibitor type 1), member 1
  • Serpin E1
  • serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitortype 1), member 1

Background

PAI-1 and PAI-2 (for plasminogen activator inhibitor-1 and -2) are members of the serpin serine proteinase inhibitor family. PAI-1 and PAI-2 have been shown to regulate uPA (urokinase-type plasminogen activator) and tPA (tissue plasminogen activator), resulting in the inhibition of proteolytic activity. Members of the serpin family generally complex with their target proteinases, then disassociate slowly into cleaved species that fold into stable inactive forms. PAI-1 can fold into the inactive state without cleavage, resulting in the latent form of PAI-1. Activity can be restored to the latent form of PAI-1 through denaturation and renaturation. PAI-2 occurs in secreted and cytosolic forms through facultative polypeptide translocation. uPA is a serine proteinase that is a member of the trypsin family. It is responsible for the cleavage of plasminogen at the Arg-Val bond to produce plasmin. uPA consists of two chains designated A and B. The A chain can be cleaved, resulting in low and high molecular mass forms of uPA.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Serpin E1/PAI-1 Antibody (NBP2-37532)(1)

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FAQs for Serpin E1/PAI-1 Antibody (NBP2-37532). (Showing 1 - 1 of 1 FAQ).

  1. I have a question about PAI-1 clone 1D5 catalog NBP2-37532. For flow cytometry application do you know if this is intracellular or extracellurlar staining? 
    • Serpin E1 is a secreted protein, typically for normal flow you can not detect proteins that have already be secreted from the cell. In this case, Flow is used to detect the protein that is being made in the cells. Therefore, it would be intracellular flow.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol SERPINE1