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Serpin A5/Protein C Inhibitor Antibody (OTI2D10)

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Western Blot: Serpin A5/Protein C Inhibitor Antibody (2D10) [NBP2-01833] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Protein C inhibitor (Right lane) cDNA for 48 hrs and ...read more
Immunocytochemistry/ Immunofluorescence: Serpin A5/Protein C Inhibitor Antibody (OTI2D10) [NBP2-01833] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Protein C inhibitor.
Immunohistochemistry-Paraffin: Serpin A5/Protein C Inhibitor Antibody (2D10) [NBP2-01833] - Staining of paraffin-embedded Human tonsil using anti-Protein C inhibitor mouse monoclonal antibody.
Flow Cytometry: Serpin A5/Protein C Inhibitor Antibody (2D10) [NBP2-01833] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Protein C ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC
Clone
OTI2D10
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.75 mg/ml

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Serpin A5/Protein C Inhibitor Antibody (OTI2D10) Summary

Immunogen
Full length human recombinant protein of human SERPINA5(NP_000615) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
SERPINA5
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:2000
Theoretical MW
45.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
0.75 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Serpin A5/Protein C Inhibitor Antibody (OTI2D10)

  • Acrosomal serine protease inhibitor
  • antitrypsin), member 5
  • member 5
  • PAI-3
  • PAI3PLANH3
  • PCI
  • PCIplasminogen activator inhibitor III
  • Plasminogen activator inhibitor 3
  • plasminogen activator inhibitor-3
  • PROCIplasma serine protease inhibitor
  • Protein C inhibitor
  • serine (or cysteine) proteinase inhibitor, clade A (alpha-1 antiproteinase
  • Serpin A5
  • serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin)
  • SERPNA5

Background

PAI-1, PAI-2 and PAI-3 (plasminogen activator inhibitor-1, -2 and -3) are members of the serpin serine proteinase inhibitor family. PAI-1 and PAI-2 regulate uPA (urokinase-type plasminogen activator) and TPA (tissue plasminogen activator), resulting in the inhibition of proteolytic activity. Members of the serpin family generally complex with their target proteinases, then disassociate slowly into cleaved species that fold into stable inactive forms. PAI-1 can fold into the inactive state without cleavage resulting in the latent form of PAI-1. Activity can be restored to the latent form of PAI-1 through denaturation and renaturation. PAI-2 occurs in secreted and cytosolic forms through facultative polypeptide translocation. PAI-3 inhibits plasminogen activators as well as activated protein C. PAI-3 is secreted in plasma, but is also expressed in liver.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Serpin A5/Protein C Inhibitor Antibody (NBP2-01833). (Showing 1 - 2 of 2 FAQ).

  1. I would like to know if your Serpin A5/Protein C Inhibitor Antibody (2D10) is it the same antibody required for plasma serine protease inhibitor.
    • Our Serpin A5/Protein C Inhibitor antibody (2D10) with catalogue number NBP2-01833 was raised against full length recombinant human SERPINA5. SERPINA5 is also known as plasma serine protease inhibitor.
  2. Can I please have a concentration of NBP2-01833, Serpin A5/Protein C Inhibitor Antibody (2D10) 0.1 ml, lot# A01.
    • Lot A01 is at 0.75mg/ml.

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Bioinformatics

Gene Symbol SERPINA5
Entrez