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Serotonin N-acetyltransferase [p Thr29] Antibody - BSA Free

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Western Blot: Serotonin N-acetyltransferase [p Thr29] Antibody [NB120-3439] - Analysis of phospho AA-NAT in transfected 293T cell lysate.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

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Serotonin N-acetyltransferase [p Thr29] Antibody - BSA Free Summary

Immunogen
Synthetic Peptide: C(24) Q R R H T(p) L P A S(33)
Modification
p Thr29
Specificity
NB120-3439 detects phospho serotonin N-acetyltransferase (AA-NAT) from rat tissues.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
AANAT
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Western Blot 0.5 ug/ml
Application Notes
WB: Detects an approx. 23 kDa protein representing phospho AA-NAT in rat pineal gland whole protein extract.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Serotonin N-acetyltransferase [p Thr29] Antibody - BSA Free

  • AA-NAT
  • aralkylamine N-acetyltransferaseEC 2.3.1.87
  • arylalkylamine N-acetyltransferase
  • Serotonin acetylase
  • SNATserotonin N-acetyltransferase

Background

Pineal gland Serotonin N-acetyltransferase (Arylalkylamine N-acetyltransferase, AA-NAT) is an ~23 kDa protein which is the rate-limiting enzyme in melatonin synthesis. Melatonin serves as the hormonal signal of the daily light / dark cycle, or body clock. The nocturnal oscillation in enzyme activity ranges by 10- to 100-fold depending on species among vertebrates. Studies have shown that AA-NAT is regulated by cAMP at both the mRNA and protein levels. AA-NAT has putative protein kinase A (PKA) sites at Thr 29 and Ser 206. Phosphorylation of AA-NAT at these residues might play a role in protein inactivation via proteasome-dependent degradation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol AANAT