Separase Antibody (XJ11-4D7)

In normal cellular proliferation, the maintenance of constant centriole numbers is strongly associated with cell cycle process and the disengagement of mother-daugther centriole (a requirement for duplication of centriole) is facilitated by separase (ESPL1, a cysteine endopeptidase) mediated proteolytic cleavage of cohesin, a "glue" protein complex which is also essential to sister-chromatide cohesion. Separase is a caspase-like protease which plays a key role in chromosome segregation by cleaving SCC1/RAD21 subunit of the cohesin complex at the onset of anaphase. During most of the cell cycle phases, separase is kept inactivated, and its proteolytic activity is tightly regulated by multiple inhibitory mechanisms combining securin binding, CyclinB1/Cdk1 mediated serine residue phosphorylation (pSer1226), PP2A binding and autocatalytic cleavage. Ectopic activation of separase proteolytic activity results in premature sister-chromatide separation/centriole disengagement and separase overexpression has been shown to induce aneuploidy and carcinogenesis.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 1 application: WB.

Showing Publications 1 - 4 of 4.

Publications using NB100-330	Applications	Species
Kim H, Jeon Y, Ha G et al. Functional interaction between BubR1 and securin in an anaphase-promoting complex/cyclosomeCdc20-independent manner. Cancer Res. 2009-01-01 [PMID: 19117984] (WB, Human)	WB	Human
Fujita T, Epperly M, Zou H et al. Regulation of the anaphase-promoting complex-separase cascade by transforming growth factor-beta modulates mitotic progression in bone marrow stromal cells. Mol Biol Cell. 2008-12-01 [PMID: 18843049] (WB, Human)	WB	Human
Sak A, Fegers I, Groneberg M Effect of separase depletion on ionizing radiation-induced cell cycle checkpoints and survival in human lung cancer cell lines. Cell Prolif. 2008-08-01 [PMID: 18616699] (WB, Human)	WB	Human
Rubinek T, Chesnokova V, Wolf I et al. Discordant Proliferation and Differentiation in Pituitary Tumor-Transforming Gene-Null Bone Marrow Stem Cells Am J Physiol Cell Physiol. 2007-09-01 [PMID: 17626243] (WB, Mouse)	WB	Mouse

Array NB100-330

• Separase Antibodies
• Separase Proteins