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Rat Secretin ELISA Kit (Colorimetric)

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ELISA: Rat Secretin ELISA Kit (Colorimetric) [NBP2-82442] - Samples were spiked with high concentrations of Rat Secretin and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
ELISA: Rat Secretin ELISA Kit (Colorimetric) [NBP2-82442] - Samples were spiked with high concentrations of Rat Secretin and diluted with Reference Standard & Sample Diluent to produce samples with values within the ...read more
ELISA: Rat Secretin ELISA Kit (Colorimetric) [NBP2-82442] - Standard Curve Reference

Product Details

Summary
Reactivity RtSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma, cell supernatant, and other biological fluids
Standard Curve Range
0.16 - 10 ng/mL
Sensitivity
0.09 ng/mL

Order Details

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Rat Secretin ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Rat Secretin in samples. No Significant cross-reactivity or interference between Rat Secretin and analogues was observed.
Standard Curve Range
0.16 - 10 ng/mL
Sensitivity
0.09 ng/mL
Assay Type
Competitive-ELISA
Inter-Assay
CV% < 4.88
Intra-Assay
CV% < 4.9
Spike Recovery
88-107%
Sample Volume
100 ul
Kit Type
ELISA Kit (Colorimetric)
Gene
SCT

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro ELISA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Stop Solution
  12. Substrate Reagent

Alternate Names for Rat Secretin ELISA Kit (Colorimetric)

  • secretin

Background

Secretin belongs to the glucagon family. This protein is an endocrine hormone and its major site of production is the endocrine S cells located in the proximal small intestinal mucosa. The release of active secretin is stimulated by either fatty acids or an acidic pH in the duodenum. This hormone stimulates the secretion of bicarbonate-rich pancreatic fluids and has also been shown to regulate the growth and development of the stomach, small intestine, and pancreas. Secretin deficiency has been implicated in autistic syndrome, suggesting that the hormone could have a neuroendocrine function in addition to its role in digestion. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Product PDFs

  SDS
  Rat Secretin ELISA Kit (Colorimetric) Manual
  PDF Datasheet
  Request CofA

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Bioinformatics

Gene Symbol SCT