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SEC23IP Antibody

Images

 
Genetic Strategies: Western Blot: SEC23IP Antibody [NBP1-82456] - Analysis in A-549 cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented. Loading ...read more
Western Blot: SEC23IP Antibody [NBP1-82456] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Immunocytochemistry/ Immunofluorescence: SEC23IP Antibody [NBP1-82456] - Staining of human cell line A-431 shows positivity in vesicles. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: SEC23IP Antibody [NBP1-82456] - Staining of human tonsil shows moderate granular cytoplasmic positivity in germinal center cells.
Immunohistochemistry-Paraffin: SEC23IP Antibody [NBP1-82456] - Staining of human skeletal muscle shows no positivity in myocytes as expected.
Immunohistochemistry-Paraffin: SEC23IP Antibody [NBP1-82456] - Staining of human stomach shows strong granular cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: SEC23IP Antibody [NBP1-82456] - Staining of human testis shows moderate granular cytoplasmic positivity in cells in seminiferous ducts.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, KD
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
     

Genetic Strategies

   

Order Details

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SEC23IP Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: HLELKESLSRMGSDLKQGFISSLKSAWQTLNEFARAHTSSTQLQEELEKVANQIKEEEEKQVVEAEKVVESPDFSKDEDYLGKVGML
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SEC23IP
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Knockdown Validated
  • Western Blot 0.04 - 0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
SEC23IP Protein (NBP1-82456PEP)
Publications
Read Publication using NBP1-82456.

Reactivity Notes

Reactivity reported in scientific literature (PMID: 23435261)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for SEC23IP Antibody

  • P125
  • P125A
  • SEC23 interacting protein
  • SEC23-interacting protein

Background

COPII-coated vesicles are involved in protein transport from the endoplasmic reticulum to the Golgi apparatus. The protein encoded by this gene was identified by its interaction with a mouse protein similar to yeast Sec23p, an essential component of the COPII. This protein shares significant similarity with phospholipid-modifying proteins, especially phosphatidic acid preferring-phospholipase A1. Overexpression of this protein has been shown to cause disorganization of the endoplasmic reticulum-Golgi intermediate compartment and Golgi apparatus, which suggests its role in the early secretory pathway.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for SEC23IP Antibody (NBP1-82456)(1)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for SEC23IP Antibody (NBP1-82456) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol SEC23IP