SDHA Antibody (1084056) [Unconjugated] Summary
Immunogen |
Synthetic Peptide Accession # P31040 |
Specificity |
Detects a synthetic peptide corresponding to residues near amino acid 610 of human SDHA protein by Direct ELISA. |
Source |
N/A |
Isotype |
IgG2b |
Clonality |
Monoclonal |
Host |
Mouse |
Purity Statement |
Protein A or G purified from hybridoma culture supernatant |
Innovator's Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase. |
Applications/Dilutions
Dilutions |
- Immunocytochemistry 3-25 ug/mL
- Immunohistochemistry 3-25 ug/mL
- Simple Western 20 ug/mL
- Western Blot 1 ug/mL
|
Packaging, Storage & Formulations
Storage |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
|
Buffer |
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. |
Reconstitution Instructions |
Reconstitute lyophilized material at 0.2 mg/ml in sterile PBS. For liquid material, refer to CoA for concentration. |
Notes
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for SDHA Antibody (1084056) [Unconjugated]
Background
SDHA is 73 kDa protein located on the inner membrane of the
mitochondria which participates in both the citric acid cycle and the
respiratory chain. SDHA is one subunit of the SDH complex, which contains 4
total subunits, that are encoded by nuclear genes. All the SDH genes are involved in the
tumorigenesis of different types of cancers. Germline SDHA mutations are a driver for multiple types of cancers.
- Horsefield R, Yankovskaya V, Sexton G, Whittingham W, Shiomi K, Omura S,
Byrne B, Cecchini G, Iwata S. Structural and computational analysis of the
quinone-binding site of complex II (succinate-ubiquinone oxidoreductase): a
mechanism of electron transfer and proton conduction during ubiquinone
reduction. J Biol Chem. 2006 Mar 17;281(11):7309-16. doi:
10.1074/jbc.M508173200. Epub 2005 Dec 27. PMID: 16407191.
- Schipani A, Nannini M,
Astolfi A, Pantaleo MA. SDHA Germline Mutations in
SDH-Deficient GISTs: A Current Update. Genes (Basel). 2023 Mar 4;14(3):646.
doi: 10.3390/genes14030646. PMID: 36980917; PMCID: PMC10048394.
- Dubard Gault M, Mandelker D,
DeLair D, Stewart CR, Kemel Y, Sheehan MR, Siegel B, Kennedy J, Marcell V,
Arnold A, Al-Ahmadie H, Modak S, Robson M, Shukla N, Roberts S, Vijai J, Topka
S, Kentsis A, Cadoo K, Carlo M, Latham Schwark A, Reznik E, Dinatale R,
Hechtman J, Borras Flores E, Jairam S, Yang C, Li Y, Bayraktar EC,
Ceyhan-Birsoy O, Zhang L, Kohlman W, Schiffman J, Stadler Z, Birsoy K, Kung A,
Offit K, Walsh MF. Germline SDHA mutations in children and
adults with cancer. Cold Spring Harb Mol Case Stud. 2018 Aug 1;4(4):a002584.
doi: 10.1101/mcs.a002584. PMID: 30068732; PMCID: PMC6071569.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
guaranteed for 1 year from date of receipt.
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