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SDHA Antibody (1084056) [Unconjugated]

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Western Blot shows lysates of HepG2 human hepatocellular carcinoma cell line and MCF‑7 human breast cancer cell line. PVDF membrane was probed with 1 µg/ml of Mouse Anti-Human SDHA Monoclonal Antibody (Catalog # ...read more
SDHA was detected in immersion fixed paraffin-embedded sections of human kidney using Mouse Anti-Human SDHA Monoclonal Antibody (Catalog # MAB11586) at 5 µg/ml for 1 hour at room temperature followed by incubation with ...read more
SDHA was detected in immersion fixed MCF‑7 human breast cancer cell line using Mouse Anti-Human SDHA Monoclonal Antibody (Catalog # MAB11586) at 8 µg/ml for 3 hours at room temperature. Cells were stained using ...read more
Simple Western shows lysates of HepG2 human hepatocellular carcinoma cell line, loaded at 1 mg/ml. A specific band was detected for SDHA at approximately 63 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human SDHA ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, IHC, ICC/IF
Clone
1084056
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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Catalog# & Formulation Size Price
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SDHA Antibody (1084056) [Unconjugated] Summary

Immunogen
Synthetic Peptide
Accession # P31040
Specificity
Detects a synthetic peptide corresponding to residues near amino acid 610 of human SDHA protein by Direct ELISA.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry 3-25 ug/mL
  • Immunohistochemistry 3-25 ug/mL
  • Simple Western 20 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Instructions
Reconstitute lyophilized material at 0.2 mg/ml in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SDHA Antibody (1084056) [Unconjugated]

  • CMD1GG
  • flavoprotein subunit of complex II
  • FP
  • MC2DN1
  • NDAXOA
  • PGL5
  • PPGL5
  • SDH1
  • SDH2
  • SDHA
  • SDHF
  • succinate dehydrogenase complex, subunit A, flavoprotein (Fp)

Background

SDHA is 73 kDa protein located on the inner membrane of the mitochondria which participates in both the citric acid cycle and the respiratory chain. SDHA is one subunit of the SDH complex, which contains 4 total subunits, that are encoded by nuclear genes.  All the SDH genes are involved in the tumorigenesis of different types of cancers. Germline SDHA mutations are a driver for multiple types of cancers.
  1. Horsefield R, Yankovskaya V, Sexton G, Whittingham W, Shiomi K, Omura S, Byrne B, Cecchini G, Iwata S. Structural and computational analysis of the quinone-binding site of complex II (succinate-ubiquinone oxidoreductase): a mechanism of electron transfer and proton conduction during ubiquinone reduction. J Biol Chem. 2006 Mar 17;281(11):7309-16. doi: 10.1074/jbc.M508173200. Epub 2005 Dec 27. PMID: 16407191. 
  2. Schipani A, Nannini M, Astolfi A, Pantaleo MA. SDHA Germline Mutations in SDH-Deficient GISTs: A Current Update. Genes (Basel). 2023 Mar 4;14(3):646. doi: 10.3390/genes14030646. PMID: 36980917; PMCID: PMC10048394.
  3. Dubard Gault M, Mandelker D, DeLair D, Stewart CR, Kemel Y, Sheehan MR, Siegel B, Kennedy J, Marcell V, Arnold A, Al-Ahmadie H, Modak S, Robson M, Shukla N, Roberts S, Vijai J, Topka S, Kentsis A, Cadoo K, Carlo M, Latham Schwark A, Reznik E, Dinatale R, Hechtman J, Borras Flores E, Jairam S, Yang C, Li Y, Bayraktar EC, Ceyhan-Birsoy O, Zhang L, Kohlman W, Schiffman J, Stadler Z, Birsoy K, Kung A, Offit K, Walsh MF. Germline SDHA mutations in children and adults with cancer. Cold Spring Harb Mol Case Stud. 2018 Aug 1;4(4):a002584. doi: 10.1101/mcs.a002584. PMID: 30068732; PMCID: PMC6071569.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

Isotype Controls

Additional SDHA Products

Blogs on SDHA.

SDHA - oxidative enzyme in the citric acid cycle
Succinate dehydrogenase is an important tetrameric protein involved in the citric acid cycle. It is localized to the inner mitochondrial membrane of cells. Succinate dehydrogenase makes up Complex II of the electron transport chain (ETC) and is re...  Read full blog post.

SDHA - An essential Krebs cycle enzyme with role in cancer and metabolism
Succinate dehydrogenase (SDH) is a highly conserved protein complex located on the inner mitochondrial membrane where it functions during the Krebs cycle by oxidizing succinate to fumarate (1). This reaction is also important for feeding electrons ...  Read full blog post.

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