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SCAP2 Antibody

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Western Blot: SCAP2 Antibody [NBP2-47289] - Analysis in control (vector only transfected HEK293T lysate) and SKAP2 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: SCAP2 Antibody [NBP2-47289] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.
Immunohistochemistry-Paraffin: SCAP2 Antibody [NBP2-47289] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.
Immunohistochemistry-Paraffin: SCAP2 Antibody [NBP2-47289] - Staining of human lymph node shows distinct cytoplasmic positivity in a subset of cells.
Orthogonal Strategies: Immunohistochemistry-Paraffin: SCAP2 Antibody [NBP2-47289] - Analysis in human lung and pancreas tissues. Corresponding SCAP2 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: SCAP2 Antibody [NBP2-47289] - Staining of human duodenum shows moderate cytoplasmic positivity in lymphoid cells.
Independent Antibodies: Immunohistochemistry-Paraffin: SCAP2 Antibody [NBP2-47289] - Staining of human duodenum, lung, lymph node and pancreas using Anti-SCAP2 antibody NBP2-47289 (A) shows similar protein ...read more
Immunohistochemistry-Paraffin: SCAP2 Antibody [NBP2-47289] - Staining of human lung shows moderate cytoplasmic positivity in macrophages.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

SCAP2 Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: LPEEIRNLLADVETFVADILKGENLSKKAKEKRESLIKKIKDVKSIYLQEFQDKGDAEDGEEYDDPFAGPPDTISLASERYDKDDEAPSDGAQFPPIAAQDLPFVLKAGYLEKRRKDHSFLGFEWQKRWCALSKTVFYY
Predicted Species
Mouse (94%), Rat (91%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SKAP2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
SCAP2 Protein (NBP2-47289PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for SCAP2 Antibody

  • Fyn-associated phosphoprotein SKAP55 homologue
  • PRAP
  • RA70Pyk2/RAFTK-associated protein
  • Retinoic acid-induced protein 70
  • SAPSSKAP55 homolog
  • SCAP2MGC33304
  • SKAP55RSKAP-55HOM
  • SKAP-HOMMGC10411
  • src family associated phosphoprotein 2
  • Src family-associated phosphoprotein 2
  • src kinase associated phosphoprotein 2
  • src kinase-associated phosphoprotein 2
  • Src kinase-associated phosphoprotein 55-related protein
  • src kinase-associated phosphoprotein of 55-related protein
  • Src-associated adapter protein with PH and SH3 domains
  • src-associated adaptor protein

Background

SCAP2 is encoded by this gene belongs to the src family kinases. This protein is similar to the src kinase associated phosphoprotein 1. It is an adaptor protein that is thought to play an essential role in the src signaling pathway in various cells. It inhibits PTK2B/RAFTK activity and regulates alpha-synuclein phosphorylation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol SKAP2