I need to radiolabel (iodinate) the SAT1 antibody. Which one should I use?

Name: SAT1 Antibody [CoraFluor&#8482; 1]
Brand: Novus Biologicals
SKU: NB110-41622CL1
Price: 899 USD

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Accepted Answer

I would recommend NB110-41622 for you as it is a well validated antibody with very clean QC validation data, multiple applications/broad species reactivity. Additionally, this antibody has been cited by Liao et al. in The Journal of Biological Chemistry 2009;284(12):8174-8184 (https://www.jbc.org/article/S0021-9258(20)32529-1/fulltext).

At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Reactivity	Hu, Mu, Rt, Po, Bv, Ch, Gt, XpSpecies Glossary
Applications	WB, IHC
Clonality	Polyclonal
Host	Rabbit
Conjugate	CoraFluor 1

Catalog# & Conjugate	Size	Price
Alexa Fluor 350 NB110-41622AF350

Alexa Fluor 405 NB110-41622AF405

Alexa Fluor 488 NB110-41622AF488

Alexa Fluor 532 NB110-41622AF532

Alexa Fluor 594 NB110-41622AF594

Alexa Fluor 647 NB110-41622AF647

Alexa Fluor 700 NB110-41622AF700

Alexa Fluor 750 NB110-41622AF750

Allophycocyanin NB110-41622APC

Biotin NB110-41622B

DyLight 350 NB110-41622UV

DyLight 405 NB110-41622V

DyLight 488 NB110-41622G

DyLight 550 NB110-41622R

DyLight 594 NB110-41622DL594

DyLight 650 NB110-41622C

DyLight 680 NB110-41622FR

DyLight 755 NB110-41622IR

FITC NB110-41622F

HRP NB110-41622H

Janelia Fluor 525 NB110-41622JF525

Janelia Fluor 549 NB110-41622JF549

Janelia Fluor 646 NB110-41622JF646

Janelia Fluor 669 NB110-41622JF669

PE NB110-41622PE

PerCP NB110-41622PCP

Unconjugated NB110-41622

mFluor Violet 450 SE NB110-41622MFV450

mFluor Violet 500 SE NB110-41622MFV500

mFluor Violet 610 SE NB110-41622MFV610

Catalog# & Formulation	Size	Price
Standard Formulation NB110-41622

BSA Free NB110-41622SS

BSA Free NB110-41622

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB110-41622AF350
Alexa Fluor 405	NB110-41622AF405
Alexa Fluor 488	NB110-41622AF488
Alexa Fluor 532	NB110-41622AF532
Alexa Fluor 594	NB110-41622AF594
Alexa Fluor 647	NB110-41622AF647
Alexa Fluor 700	NB110-41622AF700
Alexa Fluor 750	NB110-41622AF750
Allophycocyanin	NB110-41622APC
Biotin	NB110-41622B
DyLight 350	NB110-41622UV
DyLight 405	NB110-41622V
DyLight 488	NB110-41622G
DyLight 550	NB110-41622R
DyLight 594	NB110-41622DL594
DyLight 650	NB110-41622C
DyLight 680	NB110-41622FR
DyLight 755	NB110-41622IR
FITC	NB110-41622F
HRP	NB110-41622H
Janelia Fluor 525	NB110-41622JF525
Janelia Fluor 549	NB110-41622JF549
Janelia Fluor 646	NB110-41622JF646
Janelia Fluor 669	NB110-41622JF669
PE	NB110-41622PE
PerCP	NB110-41622PCP
Unconjugated	NB110-41622
mFluor Violet 450 SE	NB110-41622MFV450
mFluor Violet 500 SE	NB110-41622MFV500
mFluor Violet 610 SE	NB110-41622MFV610

SAT1 Antibody [CoraFluor™ 1]

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Bioinformatics

Description	CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
Immunogen	A synthetic peptide made to a C-terminal region within residues 100-171 of human SAT1. [Swiss-Prot# P21673]
Localization	Cytoplasm.
Isotype	IgG
Clonality	Polyclonal
Host	Rabbit
Gene	SAT1
Purity	Immunogen affinity purified
Innovator's Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator's Reward

Dilutions	Immunohistochemistry Immunohistochemistry-Paraffin Western Blot
Application Notes	Optimal dilution of this antibody should be experimentally determined.

Storage	Store at 4C in the dark. Do not freeze.
Buffer	PBS
Preservative	No Preservative
Purity	Immunogen affinity purified