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S100P Antibody [Unconjugated]

Images

 
Western blot shows lysates of HT-29 human colon adenocarcinoma parental cell line and S100P knockout HT-29 cell line (KO). PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human S100P Antigen Affinity-purified ...read more
S100P was detected in immersion fixed paraffin-embedded sections of human stomach using Goat Anti-Human S100P Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2957) at 0.3 µg/mL for 1 hour at room temperature ...read more
Genetic Strategies: S100P was detected in immersion fixed HT-29 human colon adenocarcinoma cell line but is not detected in S100P knockout (KO) HT-29 Human Cell Line cell line using Goat Anti-Human S100P Antigen ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, KO
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH
Validated by:
     

Genetic Strategies

   

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Catalog# & Formulation Size Price
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S100P Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human S100P
Met1-Lys95
Accession # P25815
Specificity
Detects human S100P in direct ELISAs and Western blots. In Western blots, less than 5% cross-reactivity with recombinant human (rh) S100B and rhS100A10 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
S100P
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 0.3-15 ug/mL
  • Knockout Validated
  • Western Blot 0.5 ug/mL
Publications
Read Publications using
AF2957 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for S100P Antibody [Unconjugated]

  • MIG9
  • migration-inducing gene 9
  • Protein S100-E
  • S100 calcium binding protein P
  • S100 calcium-binding protein Pprotein S100-P
  • S100E
  • S100P

Background

S100P is a member of the S100 protein family, whose members are calcium-binding proteins containing two characteristic E-F hand motifs. S100P proteins exist as homodimers or heterodimers (e.g. with S100A1). They interact with target proteins in a Ca++-dependent manner to regulate cell functions. S100P is localized intracellularly but can also be released from cells to act extracellularly. The amino acid sequence of human S100P is 47% identical to that of rat S100P.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for S100P Antibody (AF2957)(8)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 5 applications: Functional Assay, ICC, IHC, IHC-P, Western Blot.


Filter By Application
Functional Assay
(2)
ICC
(1)
IHC
(1)
IHC-P
(1)
Western Blot
(2)
All Applications
Filter By Species
Human
(7)
All Species
Showing Publications 1 - 8 of 8.
Publications using AF2957 Applications Species
Lancaster, T;Tabrizi, MEA;Repici, M;Gupta, J;Gross, SR; An Extracellular/Membrane-Bound S100P Pool Regulates Motility and Invasion of Human Extravillous Trophoblast Lines and Primary Cells Biomolecules 2023-08-09 [PMID: 37627296] (Functional Assay, Human) Functional Assay Human
TM Ismail, SR Gross, T Lancaster, PS Rudland, R Barracloug The Role of the C-Terminal Lysine of S100P in S100P-Induced Cell Migration and Metastasis Biomolecules, 2021-10-06;11(10):. 2021-10-06 [PMID: 34680103] (Functional Assay, Human) Functional Assay Human
M Akita, R Sawada, M Komatsu, N Suleman, T Itoh, T Ajiki, N Heaton, T Fukumoto, Y Zen An immunostaining panel of C-reactive protein, N-cadherin, and S100P is useful for intrahepatic cholangiocarcinoma subtyping Human pathology, 2020-12-13;0(0):. 2020-12-13 [PMID: 33321161] (IHC, Human) IHC Human
Patrícia Carneiro, Ana Margarida Moreira, Joana Figueiredo, Rita Barros, Patrícia Oliveira, Maria Sofia Fernandes et al. S100P is a molecular determinant of E-cadherin function in gastric cancer Cell Communication and Signaling 12/1/2019 [PMID: 31767037]
Liau J, Tsai J, Yuan R, Chang C, Lee H, Jeng Y Morphological subclassification of intrahepatic cholangiocarcinoma: etiological, clinicopathological, and molecular features. Mod Pathol, 2014-01-10;27(8):1163-73. 2014-01-10 [PMID: 24406866] (IHC-P, Human) IHC-P Human
Shimamoto , Seiko, Kubota , Yasuo, Yamaguchi , Fuminori, Tokumitsu , Hiroshi, Kobayashi , Ryoji Ca2+/S100 proteins act as upstream regulators of the chaperone-associated ubiquitin ligase CHIP (C terminus of Hsc70-interacting protein). J Biol Chem, 2013-01-23;288(10):7158-68. 2013-01-23 [PMID: 23344957] (Western Blot, Human) Western Blot Human
Bartling B, Rehbein G, Simm A, Silber RE, Hofmann HS Porcupine expression is associated with the expression of S100P and other cancer-related molecules in non-small cell lung carcinoma. Int. J. Oncol., 2010-04-01;36(4):1015-21. 2010-04-01 [PMID: 20198348] (Western Blot, Human) Western Blot Human
Ludvigsen M, Jacobsen C, Maunsbach AB, Honore B Identification and characterization of novel ERC-55 interacting proteins: evidence for the existence of several ERC-55 splicing variants; including the cytosolic ERC-55-C. Proteomics, 2009-12-01;9(23):5267-87. 2009-12-01 [PMID: 19927312] (ICC, Human) ICC Human

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Bioinformatics

Gene Symbol S100P
Uniprot