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Rat S100A9 ELISA Kit (Colorimetric)

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ELISA: Rat S100A9 ELISA Kit (Colorimetric) [NBP3-06957] - Samples were spiked with high concentrations of Rat S100A9 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
ELISA: Rat S100A9 ELISA Kit (Colorimetric) [NBP3-06957] - Samples were spiked with high concentrations of Rat S100A9 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range ...read more
ELISA: Rat S100A9 ELISA Kit (Colorimetric) [NBP3-06957] - Standard Curve Reference

Product Details

Summary
Reactivity RtSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, Plasma, Cell supernatant
Standard Curve Range
0.16 - 10 ng/mL
Sensitivity
0.09 ng/mL

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Rat S100A9 ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Rat S100A9 in samples. No significant cross-reactivity or interference between Rat S100A9 and analogues was observed.
Standard Curve Range
0.16 - 10 ng/mL
Sensitivity
0.09 ng/mL
Assay Type
Sandwich-ELISA
Inter-Assay
5.77
Intra-Assay
4.92
Spike Recovery
89-109 %
Sample Volume
100 ul
Kit Type
ELISA Kit (Colorimetric)
Gene
S100A9

Applications/Dilutions

Dilutions
  • ELISA
  • Sandwich ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro ELISA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Stop Solution
  12. Substrate Reagent

Alternate Names for Rat S100A9 ELISA Kit (Colorimetric)

  • CAGBMigration inhibitory factor-related protein 14
  • Calgranulin B
  • calgranulin-B
  • Calprotectin L1H subunit
  • CFAGMRP-14,60B8AG
  • CGLB
  • L1AG
  • LIAG
  • MAC387
  • MIF
  • MRP-14
  • MRP14Leukocyte L1 complex heavy chain
  • NIF
  • P14
  • protein S100-A9
  • S100 calcium binding protein A9 (calgranulin B)
  • S100 calcium binding protein A9
  • S100 calcium-binding protein A9 (calgranulin B)
  • S100 calcium-binding protein A9
  • S100A9

Background

S100A9 is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and are involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100A9 may function in the inhibition of casein kinase, and altered expression is associated with the disease cystic fibrosis. Overexpression of S100A9 has also recently been linked to a poor prognosis with invasive ductal carcinoma of the breast.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Rat S100A9 ELISA
Kit (Colorimetric)
NBP3-06957
Rat S100A9 ELISA Kit (Colorimetric)
Species: Rt
Applications: ELISA

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Product General Protocols

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FAQs for S100A9 ELISA Kit (NBP3-06957). (Showing 1 - 2 of 2 FAQ).

  1. Have any of your S100A9 antibodies been tested for use in neutralization, or blocking assays, on human samples?
    • Unfortunately at this time we have not tested, or received customer feedback on our S100A9 antibodies for use in blocking or neutralizing assays. If you were planning on testing any of our S100A9 antibodies for neutralizing or blocking capabilities, we would recommend our Innovator’s Reward Program. Under the terms of this program we would be happy to provide a credit for a free vial in exchange for new data on this previously untested or reported application. Please submit this data in the form of an online review. Additional <a href="http://www.novusbio.com/support/innovators-reward.html" target="_self">Innovators Reward Program</a> information can be found using this link.
  2. We would like to stain paraffin embedded human intestinal tissue for Calprotectin. One question will be which cells express the majority of Calprotectin - invading neutrophils vs epithelium for example. The antibody list you provide is quite extensive. Which antibody would you recommend for our purpose? Which tissue would you recommend as positive control.
    • Nine of our antibodies to Calprotectin have been validated for IHC-P with human tissue, and you can see all of these products. A number of our IHC images were generated using human spleen samples, and as such this may be a good choice of a positive control tissue. We do sell a human spleen slide product, which is suitable for IHC-P. Unfortunately I do not have an in-depth knowledge of Calprotectin, however the image shown for our antibody with catalogue number NBP1-02826 demonstrates clear staining of neutrophils. The following paper also suggests that the protein is abundant in neutrophils: PMID 11435495.

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  Rat S100A9 ELISA Kit (Colorimetric) Manual
  PDF Datasheet
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Bioinformatics

Gene Symbol S100A9