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Exosome component 10 Antibody

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Western Blot: Exosome component 10 Antibody [NBP1-82448] - Analysis in control (vector only transfected HEK293T lysate) and EXOSC10 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in ...read more
Immunohistochemistry-Paraffin: Exosome component 10 Antibody [NBP1-82448] - Staining of human Skin shows moderate nuclear positivity in squamous epithelial cells.
Genetic Strategies: Western Blot: Exosome component 10 Antibody [NBP1-82448] - Analysis in HEK293 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-EXOSC10 antibody. ...read more
Immunohistochemistry-Paraffin: Exosome component 10 Antibody [NBP1-82448] - Staining of human esophagus shows strong nucleolar positivity in superficial squamous epithelial cells.
Immunohistochemistry-Paraffin: Exosome component 10 Antibody [NBP1-82448] - Staining of human Cerebral cortex shows moderate nuclear positivity in neuronal cells.
Immunohistochemistry-Paraffin: Exosome component 10 Antibody [NBP1-82448] - Staining of human Liver shows very weak nuclear positivity in hepatocytes.
Immunohistochemistry-Paraffin: Exosome component 10 Antibody [NBP1-82448] - Staining of human Testis shows moderate nuclear positivity in cells in seminiferous ducts and leydig cells.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC, KD
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
     

Genetic Strategies

   

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Exosome component 10 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: KVTELEDKFDLLVDANDVILERVGILLDEASGVNKNQQPVLPAGLQVPKTVVSSWNRKAAEYGKKAKSETFRLLHA
Predicted Species
Mouse (93%), Rat (93%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
EXOSC10
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:20 - 1:50
  • Immunohistochemistry-Paraffin 1:20-1:50
  • Knockdown Validated
  • Western Blot 1:100 - 1:250
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Control Peptide
Exosome component 10 Protein (NBP1-82448PEP)
Publications
Read Publication using NBP1-82448.

Reactivity Notes

Reactivity reported in scientific literature (PMID: 23435261)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Exosome component 10 Antibody

  • autoantigen PM-SCL
  • EC 3.1.13.-
  • exosome component 10
  • P100 polymyositis-scleroderma overlap syndrome-associated autoantigen
  • p2
  • p3
  • p4
  • PM/Scl-100Autoantigen PM/Scl 2
  • PM-Scl
  • PMSCL2PMSCL
  • polymyositis/scleroderma autoantigen 2 (100kD)
  • Polymyositis/scleroderma autoantigen 2
  • polymyositis/scleroderma autoantigen 2, 100kDa
  • RRP6
  • Rrp6p
  • RRP6Polymyositis/scleroderma autoantigen 100 kDa

Background

Part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. Involved in nonsense-mediated decay (NMD) of mRNAs containing premature stop codons. Component of the exosome 3'->5' exoribonuclease complex, a complex that degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions. Required for the 3'processing of the 7S pre-RNA to the mature 5.8S rRNA. Could be an exonuclease . Plays a role in replication-dependent histone mRNA degradation. Mediates the association of MTR4, C1D and MPP6 wth the exosome and this complex is required for the maturation of 5.8S rRNA. Required for nucleolar localization of C1D

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Exosome component 10 Antibody (NBP1-82448)(1)

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Video Protocols

WB Video Protocol

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Bioinformatics

Gene Symbol EXOSC10