Have any of your S100A9 antibodies been tested for use in neutralization, or blocking assays, on human samples?

Name: S100A9 Antibody (MRP14/840)
Brand: Novus Biologicals
SKU: NBP2-45292
Price: 429 USD

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Unfortunately at this time we have not tested, or received customer feedback on our S100A9 antibodies for use in blocking or neutralizing assays. If you were planning on testing any of our S100A9 antibodies for neutralizing or blocking capabilities, we would recommend our Innovator’s Reward Program. Under the terms of this program we would be happy to provide a credit for a free vial in exchange for new data on this previously untested or reported application. Please submit this data in the form of an online review. Additional Innovators Reward Program information can be found using this link.

At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Reactivity	HuSpecies Glossary
Applications	Flow, ICC/IF, IHC, ICC/IF, IHC-Fr (-)
Clone	MRP14/840
Clonality	Monoclonal
Host	Mouse
Conjugate	Unconjugated
Concentration	0.2 mg/ml

Catalog# & Formulation	Size	Price
Azide and BSA Free NBP3-11441

Description	200ug/ml of antibody purified from Bioreactor Concentrate. Prepared in 1 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0 mg/ml. (NBP3-11441) Antibody with azide - store at 2 to 8C.
Immunogen	Recombinant human S100A9 protein (Uniprot: P06702)
Localization	Cytoplasmic
Marker	Macrophage Marker
Specificity	Recognizes a protein of14kDa, identified as MRP-14 (also known as Calgranulin B or S100AA9). It comprises 60% of the cytoplasmic protein fraction of circulating polymorphonuclear granulocytes and is also found in monocytes, macrophages and ileal tissue eosinophils. Peripheral blood monocytes carry the antigen extra- and intracellularly, neutrophils only intracellularly. It is a potent chemotactic factor for neutrophils. Plasma concentrations are elevated in diseases associated with increased neutrophil activity, like inflammatory bowel disease. Granulocytes terminate their existence after transmigration through the intestinal wall. Therefore, it is also detectable in feces. Elevated levels have been observed in body fluids such as plasma, saliva, gingival crevicular fluid, stools, and synovial fluid during infection and inflammatory conditions. This monoclonal antibody reacts with neutrophils, monocytes, and macrophages, and has been shown as an important marker for identifying macrophages in tissue sections.
Isotype	IgM Kappa
Clonality	Monoclonal
Host	Mouse
Gene	S100A9
Purity	Protein L or PEG purified
Innovator's Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator's Reward

Dilutions	Flow Cytometry 1-2 ug/million cells Immunocytochemistry/ Immunofluorescence 1-2 ug/ml Immunofluorescence 0.5 - 1.0 ug/ml Immunohistochemistry Immunohistochemistry-Paraffin 1-2 ug/ml
Application Notes	Immunohistochemistry (Formalin-fixed): Not suitable for frozen tissues. 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined.
Theoretical MW	14 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

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Storage	Store at 4C.
Buffer	1 mM PBS with 0.05% BSA
Preservative	0.05% Sodium Azide
Concentration	0.2 mg/ml
Purity	Protein L or PEG purified

Gene Symbol	S100A9
Uniprot	P06702() P06702()