S100A8/S100A9 Heterodimer Antibody (27E10)

The monoclonal antibody 27E10 recognizes an epitope specific for the S100A8/A9 heterocomplex that is not exposed on the individual subunits S100A8 (MRP8, calgranulin-A) or S100A9 (MRP14, calgranulin-B). The calcium-binding migration inhibitory factor-related proteins, MRP-8 (S100A8) and MRP-14 (S100A9) belong to the S100 protein family. The expression of these proteins is largely confined to the cytosol of neutrophils and monocytes. The complex formation of these proteins is a calcium-dependent process. The S100A8/A9 heterocomplex, also called MRP-8/MRP-14 complex or calprotectin, comprises 60% of the cytoplasmic protein fraction of circulating polymorphonuclear granulocytes and is also found in monocytes, macrophages and ileal tissue eosinophils. Peripheral blood monocytes carry the antigen extra- and intracellularly, neutrophils only intracellularly. The S100A8/A9 complex has antibacterial, antifungal, immunomodulating and antiproliferative effects. Besides this it is a potent chemotactic factor for neutrophils. Plasma concentrations are elevated in diseases associated with increased neutrophil activity, like inflammatory bowel disease. Granulocytes terminate their existence after transmigration through the intestinal wall. Therefore calprotectin is also detectable in feces. Elevated levels of calprotectin have been observed in body fluids such as plasma, saliva, gingival crevicular fluid, stools, and synovial fluid during infection and inflammatory conditions. The monoclonal antibody 27E10 can be used for early detection of inflammatory macrophages, for the characterization of tumorous tissues and the monitoring of peripheral blood cell cultures. The antibody 27E10 does not react with lymphocytes or platelets.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: IF/IHC, IHC-P.

Showing Publications 1 - 9 of 9.

Publications using NBP1-60157	Applications	Species
Li Z, McGinn O, Wu Y et al. ESR1 mutant breast cancers show elevated basal cytokeratins and immune activation Nature communications 2022-04-19 [PMID: 35440136] (IHC-P, Human)	IHC-P	Human
D'Amico F, Granata M, Skarmoutsou E et al. Biological therapy downregulates the heterodimer S100A8/A9 (calprotectin) expression in psoriatic patients. Inflamm. Res. 2018-03-31 [PMID: 29605872] (IF/IHC, Human)	IF/IHC	Human
Molin S, Merl J, Dietrich KA et al. The hand eczema proteome: imbalance of epidermal barrier proteins. Br. J. Dermatol. 2014-09-21 [PMID: 25244099] (IHC-P, Human) Details: Filaggrin family member 2/Filaggrin2 or FLG2 antibody used for IHC on formalin-fixed paraffin-embedded skin biopsy samples from human chronic hand eczema (CHE) patients. Antibody used at 1:30 dilution and detected via LSAB method with DAKO REAL? Detection System, Alkaline Phosphatase/RED, Rabbit/Mouse (Figure 2).	IHC-P	Human
Zhang H, Maric I, Diprima MJ et al. Fibrocytes represent a novel MDSC subset circulating in patients with metastatic cancer. Blood. 2013-08-15 [PMID: 23757729]
Zhu L, Okano S, Takahara M et al. Expression of S100 protein family members in normal skin and sweat gland tumors. J Dermatol Sci. 2013-06-01 [PMID: 23623205] (IHC-P, Human) Details: Psoriasin/HID5/S100A7 Clone 47C1068 (IMG-409A), IHC (paraffin): Normal human skin and sweat gland tumors, Figs 1k-O & 5A-G, Tables 1-3. Note: The 47C1068 S100A7 mAb was used at 10 ug/ml.	IHC-P	Human
Champaiboon C, Sappington KJ, Guenther BD, Ross KF, Herzberg MC. Calprotectin S100A9 calcium-binding loops I and II are essential for keratinocyte resistance to bacterial invasion. J Biol Chem;284(11):7078-90. 2009-03-13 [PMID: 19122197]
Kuhn A, Sonntag M, Sunderkotter C, Lehmann P, Vestweber D, Ruzicka T. Upregulation of epidermal surface molecule expression in primary and ultraviolet-induced lesions of lupus erythematosus tumidus. Br J Dermatol;146(5):801-9. 2002-05-01 [PMID: 12000376]
Hessian PA, Fisher L. The heterodimeric complex of MRP-8 (S100A8) and MRP-14 (S100A9) Antibody recognition, epitope definition and the implications for structure. Eur J Biochem;268(2):353-63. 2001-01-01 [PMID: 11168370]
Zwadlo G, Schlegel R, Sorg C. A monoclonal antibody to a subset of human monocytes found only in the peripheral blood and inflammatory tissues. J Immunol;137(2):512-8. 1986-07-15 [PMID: 3722815]

Array NBP1-60157

• S100A8/S100A9 Heterodimer Antibodies
• S100A8/S100A9 Heterodimer ELISAs
• S100A8/S100A9 Heterodimer Proteins and Enzymes