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Ryk Antibody (SR2178)

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Western Blot: Ryk Antibody (SR2178) [NBP3-22253] - Western blot analysis using NBP3-22253 in MCF7 cell lysate.
Western Blot: Ryk Antibody (SR2178) [NBP3-22253] - Western blot analysis using NBP3-22253 in MCF7 cell lysate.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IP
Clone
SR2178
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

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Ryk Antibody (SR2178) Summary

Additional Information
Recombinant Monoclonal Antibody
Immunogen
A synthesized peptide derived from human Ryk (Uniprot #: P34925)
Specificity
Detects endogenous levels of total Ryk
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
RYK
Purity
Affinity purified
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Applications/Dilutions

Dilutions
  • Flow Cytometry 1:50
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:50-1:200
  • Immunoprecipitation 1:50
  • Western Blot 1:1000-1:5000
Theoretical MW
72 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, pH 7.4, 150mM NaCl, 50% glycerol.
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for Ryk Antibody (SR2178)

  • D3S3195
  • EC 2.7.10
  • EC 2.7.10.1
  • hydroxyaryl-protein kinase
  • JTK5
  • JTK5A protein tyrosine kinase
  • JTK5A
  • RYK receptor-like tyrosine kinase
  • Ryk
  • RYK1
  • tyrosine-protein kinase RYK

Background

RYK (Receptor like Tyrosine kinase 1) is in the Tyrosine family of receptor kinases. It is found in the plasma membrane of heart, prostate and kidney cells. RYK contains 604 amino acids and it contains one WIF domain. It functions as a potential growth factor receptor protein tyrosine kinase. RYK are divergent from those of other classic receptor tyrosine kinases. It is an orphan receptor that contains a catalytically inactive tyrosine kinase related domain. RYK is expressed in nestin positive progenitor cells and neurons, and in a certain population of oligodendrocytes, O2A progenitor cells, and type 2 astrocytes in developing CNS.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol RYK