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ROR2 Antibody [Unconjugated]

 (13 Publications)
 
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Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and T47D human breast cancer cell line. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Human ROR2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2064) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for ROR2 at approximately 105 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and T47D human breast cancer cell line. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Human ROR2 Antigen Affinity-purified ...read more
Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for ROR2 at approximately 139 kDa (as indicated) using 10 µg/mL of Goat ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Biotin
BAF2064
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF2064-SP
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ROR2 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human ROR2
Val28-Gly403
Accession # AAA60276
Specificity
Detects human ROR2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross‑reactivity with recombinant human ROR1 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
ROR2
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Simple Western 10 ug/mL
  • Western Blot 1 ug/mL
Application Notes
In Simple Western only 10-15 uL of the recommended dilution is used per data point.
Publications
Read Publications using
AF2064 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ROR2 Antibody [Unconjugated]

  • BDB
  • BDB1
  • EC 2.7.10.1
  • MGC163394
  • neurotrophic tyrosine kinase receptor-related 2
  • Neurotrophic tyrosine kinase, receptor-related 2
  • NTRKR2
  • NTRKR2tyrosine-protein kinase transmembrane receptor ROR2
  • receptor tyrosine kinase-like orphan receptor 2
  • ROR2

Background

ROR2 is a type I receptor tyrosine kinase important in chondrocyte, cartilage and growth plate formation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ROR2 Antibody (AF2064)(13)

We have publications tested in 3 confirmed species: Human, Mouse, Primate - Chlorocebus pygerythrus (Vervet Monkey).

We have publications tested in 5 applications: ELISA Development, Flow Cytometry, ICC, Immunoprecipitation, Western Blot.


Filter By Application
ELISA Development
(1)
Flow Cytometry
(2)
ICC
(1)
Immunoprecipitation
(1)
Western Blot
(4)
All Applications
Filter By Species
Human
(6)
Mouse
(2)
Primate - Chlorocebus pygerythrus (Vervet Monkey)
(1)
All Species
Showing Publications 1 - 10 of 13. Show All 13 Publications.
Publications using AF2064 Applications Species
Kuroda, T;Yasuda, S;Matsuyama, S;Miura, T;Sawada, R;Matsuyama, A;Yamamoto, Y;Morioka, MS;Kawaji, H;Kasukawa, T;Itoh, M;Akutsu, H;Kawai, J;Sato, Y; ROR2 expression predicts human induced pluripotent stem cell differentiation into neural stem/progenitor cells and GABAergic neurons Scientific reports 2024-01-06 [PMID: 38184695] (Western Blot, Human) Western Blot Human
Rogers, S;Zhang, C;Anagnostidis, V;Liddle, C;Fishel, ML;Gielen, F;Scholpp, S; Cancer-associated fibroblasts influence Wnt/PCP signaling in gastric cancer cells by cytoneme-based dissemination of ROR2 Proceedings of the National Academy of Sciences of the United States of America 2023-09-26 [PMID: 37722040] (ICC, Human) ICC Human
Karen I Lange, Sunayna Best, Sofia Tsiropoulou, Ian Berry, Colin A Johnson, Oliver E Blacque Interpreting ciliopathy-associated missense variants of uncertain significance (VUS) in Caenorhabditis elegans Human Molecular Genetics 2022-05-19 [PMID: 34964473]
Nicholas W. Chavkin, Soichi Sano, Ying Wang, Kosei Oshima, Hayato Ogawa, Keita Horitani et al. The Cell Surface Receptors Ror1/2 Control Cardiac Myofibroblast Differentiation Journal of the American Heart Association 2021-07-06 [PMID: 34155901]
Tianzhi Huang, Angel A. Alvarez, Rajendra P. Pangeni, Craig M M. Horbinski, Songjian Lu, Sung-Hak Kim et al. A regulatory circuit of miR-125b/miR-20b and Wnt signalling controls glioblastoma phenotypes through FZD6-modulated pathways Nature Communications 10/4/2016 [PMID: 27698350]
W Wang, N Snyder, A J Worth et al. Regulation of lipid synthesis by the RNA helicase Mov10 controls Wnt5a production. Oncogenesis 1/1/2015 [PMID: 26029828]
Rasmussen N, Wright T, Brooks S, Hacker K, Debebe Z, Sendor A, Walker M, Major M, Green J, Wahl G, Rathmell W Receptor tyrosine kinase-like orphan receptor 2 (Ror2) expression creates a poised state of Wnt signaling in renal cancer. J Biol Chem, 2013-07-26;288(36):26301-10. 2013-07-26 [PMID: 23893409] (Western Blot, Human) Western Blot Human
Sivasubramanian Baskar, Adrian Wiestner, Wyndham H. Wilson, Ira Pastan, Christoph Rader Targeting malignant B cells with an immunotoxin against ROR1 mAbs 2012-05-01 [PMID: 22531447]
Ror family receptor tyrosine kinases regulate the maintenance of neural progenitor cells in the developing neocortex. J. Cell. Sci., 2012-02-10;125(0):2017-29. 2012-02-10 [PMID: 22328498] (Flow Cytometry, Mouse) Flow Cytometry Mouse
Yang J, Baskar S, Kwong KY Therapeutic Potential and Challenges of Targeting Receptor Tyrosine Kinase ROR1 with Monoclonal Antibodies in B-Cell Malignancies. PLoS ONE, 2011-06-15;6(6):e21018. 2011-06-15 [PMID: 21698301] (ELISA Development, Human) ELISA Development Human
Show All 13 Publications.

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Bioinformatics

Gene Symbol ROR2
Uniprot