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Ryk Antibody (484804)

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Western blot shows lysates of MDA-MB-453 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Ryk Antigen Affinity-purified Monoclonal Antibody (Catalog # MAB4907) followed by ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clone
484804
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

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Catalog# & Formulation Size Price
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Ryk Antibody (484804) Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human Ryk
Met1-Arg224
Accession # P34925
Specificity
Detects human Ryk in ELISA.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
RYK
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Ryk Antibody (484804)

  • D3S3195
  • EC 2.7.10
  • EC 2.7.10.1
  • hydroxyaryl-protein kinase
  • JTK5
  • JTK5A protein tyrosine kinase
  • JTK5A
  • RYK receptor-like tyrosine kinase
  • Ryk
  • RYK1
  • tyrosine-protein kinase RYK

Background

Ryk (related to tyrosine (Y) kinase; also H-RYK) is a 70-90 kDa, atypical member of the tyrosine protein kinase family. It is a type I transmembrane glycoprotein that binds Wnt, and forms a Wnt receptor complex with frizzled. In this capacity, it serves as a link between Wnt and Dishevelled. Human Ryk is 604 amino acids (aa) in length. It contains a 25 aa signal sequence and a 199 aa extracellular region (aa 26-224) that shows one WIF-1-like domain (aa 63‑191). The cytoplasmic region possesses a nonfunctional Ser/Thr protein kinase domain (aa 327-600). One isoform exists that shows a 31 aa substitution between aa’s 18-46. Over aa 47-224, human Ryk is 94% aa identical to mouse Ryk.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol RYK
Uniprot