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Ryk Antibody (484804)

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Western blot shows lysates of MDA-MB-453 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Ryk Antigen Affinity-purified Monoclonal Antibody (Catalog # MAB4907) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Ryk at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of MDA-MB-453 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Ryk Antigen Affinity-purified Monoclonal Antibody (Catalog # MAB4907) followed by ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clone
484804
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB4907-SP
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Ryk Antibody (484804) Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human Ryk
Met1-Arg224
Accession # P34925
Specificity
Detects human Ryk in ELISA.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
RYK
Purity Statement
Protein A or G purified from hybridoma culture supernatant
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Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Ryk Antibody (484804)

  • D3S3195
  • EC 2.7.10
  • EC 2.7.10.1
  • hydroxyaryl-protein kinase
  • JTK5
  • JTK5A protein tyrosine kinase
  • JTK5A
  • RYK receptor-like tyrosine kinase
  • Ryk
  • RYK1
  • tyrosine-protein kinase RYK

Background

Ryk (related to tyrosine (Y) kinase; also H-RYK) is a 70-90 kDa, atypical member of the tyrosine protein kinase family. It is a type I transmembrane glycoprotein that binds Wnt, and forms a Wnt receptor complex with frizzled. In this capacity, it serves as a link between Wnt and Dishevelled. Human Ryk is 604 amino acids (aa) in length. It contains a 25 aa signal sequence and a 199 aa extracellular region (aa 26-224) that shows one WIF-1-like domain (aa 63‑191). The cytoplasmic region possesses a nonfunctional Ser/Thr protein kinase domain (aa 327-600). One isoform exists that shows a 31 aa substitution between aa’s 18-46. Over aa 47-224, human Ryk is 94% aa identical to mouse Ryk.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol RYK
Uniprot