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Recombinant Human RUNX3/CBFA3 His Protein

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SDS-Page: Recombinant Human RUNX3/CBFA3 Protein [NBP1-44462] - 17.1 kDa (155aa) confirmed by MALDI-TOF with a purity of 95% by SDS - PAGE
SDS-Page: Recombinant Human RUNX3/CBFA3 Protein [NBP1-44462] - 17.1 kDa (155aa) confirmed by MALDI-TOF with a purity of 95% by SDS - PAGE

Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE
Concentration
0.5 mg/ml

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Recombinant Human RUNX3/CBFA3 His Protein Summary

Description
A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 53-186 of Human RUNX3/CBFA3

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MRSMVDVLAD HAGELVRTDS PNFLCSVLPS HWRCNKTLPV AFKVVALGDV PDGTVVTVMA GNDENYSAEL RNASAVMKNQ VARFNDLRFV GRSGRGKSFT LTITVFTNPT QVATYHRAIK VTVDGPREPR RHRQK
Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
RUNX3
Purity
>95%, by SDS-PAGE

Applications/Dilutions

Dilutions
  • SDS-Page
Theoretical MW
17.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20 mM Tris-HCl buffer (pH8.0), 20% glycerol, 0.1 M NaCl, 1 mM DTT
Preservative
No Preservative
Concentration
0.5 mg/ml
Purity
>95%, by SDS-PAGE

Alternate Names for Recombinant Human RUNX3/CBFA3 His Protein

  • Acute myeloid leukemia 2 protein
  • AML2
  • AML2SL3/AKV core-binding factor alpha C subunit
  • CBFA3
  • CBFA3MGC16070
  • CBF-alpha-3
  • PEA2 alpha C
  • PEA2-alpha C
  • PEBP2 alpha C
  • PEBP2A3
  • PEBP2A3FLJ34510
  • PEBP2AC
  • PEBP2-alpha C
  • runt domain, alpha subunit 3
  • runt-related transcription factor 3
  • RUNX3
  • SL3-3 enhancer factor 1 alpha C subunit
  • transcription factor AML2

Background

RUNX3, also known as runt-related transcription factor 3, belongs to the RUNX family that regulate the expression of genes involved in cellular differentiation and cell cycle progression. A heterodimer of this protein and a beta subunit forms a complex that binds to the core DNA sequence 5'-PYGPYGGT-3' found in a number of enhancers and promoters, and can either activate or suppress transcription. It also interacts with other transcription factors. It is a strong candidate as a gastric cancer tumor suppressor. Recombinant human RUNX3, fused to His-tag at N-terminus, protein was expressed in E.coli and purified by using conventional chromatography techniques

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol RUNX3